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Human TSG-6 Biotinylated Antibody

Catalog #: BAF2104
8 Citations Datasheet / COA / SDS
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BAF2104
Product Details
Citations (8)
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Human TSG-6 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TSG-6 in Western blots. In this format, approximately 70% cross-reactivity with recombinant mouse TSG‑6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSG-6 (R&D Systems, Catalog # 2104-TS)
Trp18-Leu277
Accession # P98066
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TSG‑6 (Catalog # 2104-TS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also TNFIP6) is a secreted, 35 - 39 kDa group A member of the LINK-Module superfamily of proteins (1 - 4). Human TSG-6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (amino acids 36 - 129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (amino acids 135 - 247). Link modules are alpha -helical, beta ‑sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80 - 85 kDa HC subunit from I alpha I (inter‑ alpha ‑inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation-dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10 - 12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References
  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  6. Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
Long Name
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs
7130 (Human); 21930 (Mouse)
Alternate Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

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Citations for Human TSG-6 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
    Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
    Cells
    Species: Human
    Sample Types: Cell Culture Supernates
  2. IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cells
    Authors: Muqiu Zuo, Jiankai Fang, Peiqing Huang, Shisong Liu, Pengbo Hou, Shiqing Wang et al.
    Cell Death Discovery
  3. Deletion of TNFAIP6 Gene in Human Keratinocytes Demonstrates a Role for TSG-6 to Retain Hyaluronan Inside Epidermis
    Authors: Céline Evrard, Emilie Faway, Evelyne De Vuyst, Olivier Svensek, Valérie De Glas, David Bergerat et al.
    JID Innovations
  4. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells
    Authors: R Nilson, O Lübbers, L Wei beta, K Singh, K Scharffett, M Rojewski, H Schrezenme, PH Zeplin, W Funk, L Krutzke, S Kochanek, A Kritzinger
    Viruses, 2021-06-12;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Efficacy of 3D Culture Priming is Maintained in Human Mesenchymal Stem Cells after Extensive Expansion of the Cells
    Authors: TJ Bartosh, JH Ylostalo
    Cells, 2019-09-05;8(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  6. Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes
    Authors: Tonia L Vincent
    Arthritis & rheumatology (Hoboken, N.J.), 2016-09-01;68(9):2129-40.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  7. Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It.
    Authors: Kim D, Choi H, Nishida H, Oh J, Gregory C, Lee R, Yu J, Watanabe J, An S, Bartosh T, Prockop D
    PLoS ONE, 2016-01-21;11(1):e0147553.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development (Detection)
  8. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015-01-06;10(1):e0116772.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)

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