Human TSLP Antibody Summary
Tyr29-Gln159
Accession # Q969D9
Applications
Human TSLP Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND50 is typically 0.05-0.25 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis, and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.
Product Datasheets
Citations for Human TSLP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance
Authors: B Vizio, M Boita, C Cristiano, J Mazibrada, O Bosco, A Novarino, A Prati, S Sciascia, G Rolla, L Ciuffreda, G Montrucchi, G Bellone
Oncotarget, 2018-08-28;9(67):32795-32809.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: Antibody Depletion, Neutralization -
Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion
Authors: HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li
Mol Med Rep, 2018-08-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils
Authors: B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie
Oncol Lett, 2017-10-03;14(6):7483-7488.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
Authors: Herro R, Antunes R, Aguilera A, Tamada K, Croft M
J Invest Dermatol, 2015-03-19;135(8):2109-18.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
PLoS ONE, 2014-08-29;9(8):e105498.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
Authors: Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D
J Immunol, 2014-01-22;192(4):1502-11.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin.
Authors: Schuepbach-Mallepell S, Philippe V, Bruggen M, Watanabe H, Roques S, Baldeschi C, Gaide O
J Allergy Clin Immunol, 2013-08-13;132(6):1348-57.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Thymic stromal lymphopoietin is produced by dendritic cells.
Authors: Kashyap M, Rochman Y, Spolski R
J. Immunol., 2011-06-20;187(3):1207-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood, 2010-06-10;116(12):2061-9.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis Rheum., 2008-11-01;58(11):3510-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease.
Authors: Zhang K, Shan L, Rahman MS, Unruh H, Halayko AJ, Gounni AS
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-18;293(2):L375-82.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Evidence for a Functional Thymic Stromal Lymphopoietin Signaling Axis in Fibrotic Lung Disease
Authors: Arnab Datta, Robert Alexander, Michal G. Sulikowski, Andrew G. Nicholson, Toby M. Maher, Chris J. Scotton et al.
The Journal of Immunology
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We used this antibody in an in-house ELISA along with mAb (1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.
Did not work with and without citrate retrieval. IgG used as control and a different protein marking the same cells was seen.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
