Home / TSLP / Human TSLP Antibody

Human TSLP Antibody

Catalog #: AF1398
13 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1398
AF1398-SP
Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody.
1 Image
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSLP Antibody Summary

Species Reactivity
Human
Specificity
Detects human TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse TSLP and recombinant human TSLP R is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TSLP
Tyr29-Gln159
Accession # Q969D9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TSLP (Catalog # 1398-TS)

Human TSLP Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TSLP Biotinylated Antibody (Catalog # BAF1398)

Standard: Recombinant Human TSLP Protein (Catalog # 1398-TS)

Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 0.5 ng/mL Recombinant Human TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody. View Larger

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND50 is typically 0.05-0.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis, and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

Long Name
Thymic Stromal Lymphopoietin
Entrez Gene IDs
85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names
thymic stromal lymphopoietin; TSLP

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human TSLP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. House dust mites stimulate thymic stromal lymphopoietin production in human bronchial epithelial cells and promote airway remodeling through activation of PAR2 and ERK signaling pathway
    Authors: Hsieh, YA;Hsiao, YH;Ko, HK;Shen, YL;Huang, CW;Perng, DW;Su, KC;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance
    Authors: B Vizio, M Boita, C Cristiano, J Mazibrada, O Bosco, A Novarino, A Prati, S Sciascia, G Rolla, L Ciuffreda, G Montrucchi, G Bellone
    Oncotarget, 2018-08-28;9(67):32795-32809.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Antibody Depletion, Neutralization
  3. Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion
    Authors: HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li
    Mol Med Rep, 2018-08-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils
    Authors: B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie
    Oncol Lett, 2017-10-03;14(6):7483-7488.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
    Authors: Herro R, Antunes R, Aguilera A, Tamada K, Croft M
    J Invest Dermatol, 2015-03-19;135(8):2109-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  6. DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
    Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
    PLoS ONE, 2014-08-29;9(8):e105498.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  7. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
    Authors: Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D
    J Immunol, 2014-01-22;192(4):1502-11.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  8. Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin.
    Authors: Schuepbach-Mallepell S, Philippe V, Bruggen M, Watanabe H, Roques S, Baldeschi C, Gaide O
    J Allergy Clin Immunol, 2013-08-13;132(6):1348-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Thymic stromal lymphopoietin is produced by dendritic cells.
    Authors: Kashyap M, Rochman Y, Spolski R
    J. Immunol., 2011-06-20;187(3):1207-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010-06-10;116(12):2061-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  11. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease.
    Authors: Zhang K, Shan L, Rahman MS, Unruh H, Halayko AJ, Gounni AS
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-18;293(2):L375-82.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Evidence for a Functional Thymic Stromal Lymphopoietin Signaling Axis in Fibrotic Lung Disease
    Authors: Arnab Datta, Robert Alexander, Michal G. Sulikowski, Andrew G. Nicholson, Toby M. Maher, Chris J. Scotton et al.
    The Journal of Immunology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TSLP Antibody

Average Rating: 2 (Based on 2 Reviews)

5 Star
0%
4 Star
0%
3 Star
50%
2 Star
0%
1 Star
50%

Have you used Human TSLP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human TSLP Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb (1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.

ELISA Human TSLP Antibody AF1398
Human TSLP Antibody
By avinash waghray on 03/13/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human

Did not work with and without citrate retrieval. IgG used as control and a different protein marking the same cells was seen.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Immunocytochemistry/Immunofluorescence Human TSLP Antibody AF1398