Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1223A
View all UNC13D Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled N-terminal human UNC13D peptide
Accession # Q70J99

Specificity

Detects the conventional form of human UNC13D in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human UNC13D Antibody

Detection of Human UNC13D antibody by Western Blot.

Detection of Human UNC13D by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

UNC13D antibody in NK-92 Human Cell Line by Immunocytochemistry (ICC).

UNC13D in NK‑92 Human Cell Line.

UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human UNC13D antibody by Simple WesternTM.

Detection of Human UNC13D by Simple WesternTM.

Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human UNC13D Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed NK‑92 human natural killer lymphoma cell line

Simple Western

1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line

Western Blot

0.1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB89661 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UNC13D

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues.  UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acids (aa) 40-1090 but have distinct N-terminal segments.  Human and mouse have 75% aa sequence identity throughout the region used as the immunogen.  It is unknown whether mice express the alternative isoform. 

Long Name

UNC-13 Homolog D

Alternate Names

FHL3, HLH3, HPLH3, Munc13-4

Entrez Gene IDs

201294 (Human); 70450 (Mouse); 192177 (Rat)

Gene Symbol

UNC13D

UniProt

Q70J99

Additional UNC13D Products

Product Documents for Human UNC13D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human UNC13D Antibody

For research use only

Related Research Areas

Lysosomal Enzymes

Customer Reviews for Human UNC13D Antibody (1)

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  • Human UNC13D Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 02/05/2018
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    Human UNC13D Antibody MAB89661

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Protocols

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