Human VAP-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 3.41 | 10.6 | 22.7 | 3.96 | 11.8 | 24.6 |
| Standard Deviation | 0.071 | 0.163 | 0.535 | 0.18 | 0.567 | 1.16 |
| CV% | 2.1 | 1.5 | 2.4 | 4.5 | 4.8 | 4.7 |
Recovery
The recovery of VAP-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 95-114 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: VAP-1/AOC3
VAP-1 is a type II transmembrane glycoprotein. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasation during inflammation, and also possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human VAP-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Circulating vascular adhesion protein-1(VAP-1): a possible biomarker for liver fibrosis associated with chronic hepatitis B and C
Authors: Z Öksüz, E Üçbilek, MS Serin, S Yara?, GO Temel, O Sezgin
Braz. J. Microbiol., 2020-09-21;0(0):.
Species: Human
Sample Types: Serum
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
Kidney Int Rep, 2019-06-21;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort
Authors: E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur
Int J Mol Sci, 2019-05-29;20(11):.
Species: Human
Sample Types: Serum, Synovial Fluid
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Increased Circulating VAP-1 Levels Are Associated with Liver Fibrosis in Chronic Hepatitis C Infection
Authors: M Kraemer, M Krawczyk, F Noor, F Grünhage, F Lammert, JG Schneider
J Clin Med, 2019-01-17;8(1):.
Species: Human
Sample Types: Plasma
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