Human VCAM-1/CD106 APC-conjugated Antibody

Catalog #: FAB5649A
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5649A

Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry.

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All VCAM-1/CD106 Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VCAM-1/CD106 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

Source

Monoclonal Mouse IgG_2A Clone # IE10

Purification

Protein A or G purified from ascites

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry. HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 APC-conjugated Monoclonal Antibody (Catalog # FAB5649A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human VCAM-1/CD106 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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The neovascularization effect of dedifferentiated fat cells
Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
Sci Rep, 2020-06-08;10(1):9211.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Measuring Leukocyte Adhesion to (Primary) Endothelial Cells after Photon and Charged Particle Exposure with a Dedicated Laminar Flow Chamber
Authors: N Erbeldinge, F Rapp, S Ktitareva, P Wendel, AS Bothe, T Dettmering, M Durante, T Friedrich, B Bertulat, S Meyer, MC Cardoso, S Hehlgans, F Rödel, C Fournier
Front Immunol, 2017-06-01;8(0):627.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
J. Biol. Chem., 2016-03-22;291(20):10586-601.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry