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Human VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB3572
35 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3572-SP
MAB3572-100
MAB3572-500
Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (35)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3 or recombinant mouse VEGFR2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize VEGFR2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 50 ng/mL Recombinant Human VEGFR2/KDR/Flk‑1 Fc Chimera and 10 ng/mL Recombinant Human VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGFR2/KDR/Flk-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Neutralization VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. View Larger

VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF165(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND50 is typically 10-50 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGFR2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. EMMPRIN promotes melanoma cells malignant properties through a HIF-2alpha mediated up-regulation of VEGF-receptor-2
    Authors: Bougatef F, Menashi S, Khayati F et al.
    PLoS One
  2. Modulation of miR29a improves impaired post-ischemic angiogenesis in hyperglycemia
    Authors: L Chen, E Okeke, D Ayalew, D Wang, L Shahid, AO Dokun
    Exp. Biol. Med. (Maywood), 2017-06-21;0(0):1535370217716.
  3. Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium
    Authors: Ghersi, JJ;Baldissera, G;Hintzen, J;Luff, SA;Cheng, S;Xia, IF;Sturgeon, CM;Nicoli, S;
    Nature cell biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endothelial Progenitor Cells Promote Osteosarcoma Progression and Invasiveness via AKT/PI3K Signaling
    Authors: O Doppelt-Fl, A Younis, T Tamari, O Ginesin, T Shentzer-K, D Nikomarov, G Bar-Sela, BR Coyac, YG Assaraf, H Zigdon-Gil
    Cancers, 2023-03-17;15(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development
    Authors: Shogo Tamura, Masato Mukaide, Yumi Katsuragi, Wataru Fujii, Koya Odaira, Nobuaki Suzuki et al.
    Journal of Biological Chemistry
  6. Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells
    Authors: SA Luff, JP Creamer, S Valsoni, C Dege, R Scarfò, A Dacunto, S Cascione, LN Randolph, E Cavalca, I Merelli, SA Morris, A Ditadi, CM Sturgeon
    Nature Cell Biology, 2022-04-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors
    Authors: BK Gage, S Merlin, C Olgasi, A Follenzi, GM Keller
    Cell Reports, 2022-04-05;39(1):110621.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&alpha and VEGF expression
    Authors: D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen
    Mol Med Rep, 2020-11-20;23(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells
    Authors: E Gambini, I Martinelli, I Stadiotti, MC Vinci, A Scopece, L Eramo, E Sommariva, J Resta, S Benaouadi, E Cogliati, A Paolin, A Parini, G Pompilio, F Savagner
    Int J Mol Sci, 2020-10-10;21(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
    Authors: T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil
    Cells, 2020-05-26;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Dual Action of Sulfated Hyaluronan on Angiogenic Processes in Relation to Vascular Endothelial Growth Factor-A
    Authors: L Koehler, G Ruiz-Gómez, K Balamuruga, S Rother, J Freyse, S Möller, M Schnabelra, S Köhling, S Djordjevic, D Scharnwebe, J Rademann, MT Pisabarro, V Hintze
    Sci Rep, 2019-12-02;9(1):18143.
    Species: Human
    Sample Types: Spheroid
    Applications: Neutralization
  12. Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake
    Authors: Song Yi Ko, WonJae Lee, Hilary A. Kenny, Long H. Dang, Lee M. Ellis, Eric Jonasch et al.
    Communications Biology
  13. Bone marrow characterization in COPD: a multi-level network analysis
    Authors: N Toledo-Pon, G Noell, A Jahn, A Iglesias, MA Duran, J Iglesias, A Rios, S Scrimini, R Faner, O Gigirey, A Agustí, BG Cosío
    Respir. Res., 2018-06-15;19(1):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Protease-Activated Receptor 2 Promotes Pro-Atherogenic Effects through Transactivation of the VEGF Receptor 2 in Human Vascular Smooth Muscle Cells
    Authors: Ira Indrakusuma, Tania Romacho, Jürgen Eckel
    Frontiers in Pharmacology
  15. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Endocytosis and serpentine filopodia drive blebbishield-mediated resurrection of apoptotic cancer stem cells
    Authors: Goodwin G Jinesh, Ashish M Kamat
    Cell Death Discovery
  17. The Vascular Endothelial Growth Factor Inhibitors Ranibizumab and Aflibercept Markedly Increase Expression of Atherosclerosis-Associated Inflammatory Mediators on Vascular Endothelial Cells
    Authors: Clare Arnott, Gaya Punnia-Moorthy, Joanne Tan, Sara Sadeghipour, Christina Bursill, Sanjay Patel
    PLOS ONE
  18. Identification of a Novel Pathogenic Germline KDR Variant in Melanoma
    Authors: IP Silva, A Salhi, KM Giles, M Vogelsang, SW Han, N Ismaili, KP Lui, EM Robinson, MA Wilson, RL Shapiro, A Pavlick, J Zhong, T Kirchhoff, I Osman
    Clin. Cancer Res., 2015-12-02;22(10):2377-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Decreased efficacy of drugs targeting the vascular endothelial growth factor pathway by the epigenetic silencing of FLT1 in renal cancer cells
    Authors: Jee Yeon Kim, Junha Hwang, Seo Hyun Lee, Hyo Jin Lee, Jaroslav Jelinek, Hyeseon Jeong et al.
    Clinical Epigenetics
  20. Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity.
    Authors: Fleetwood F, Klint S, Hanze M, Gunneriusson E, Frejd F, Stahl S, Lofblom J
    Sci Rep, 2014-12-17;4(0):7518.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
    Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
    PLoS ONE, 2014-04-08;9(4):e93965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Tylophorine, a phenanthraindolizidine alkaloid isolated from Tylophora indica exerts antiangiogenic and antitumor activity by targeting vascular endothelial growth factor receptor 2–mediated angiogenesis
    Authors: Sarita Saraswati, Pawan K Kanaujia, Shakti Kumar, Ranjeet Kumar, Abdulqader A Alhaider
    Molecular Cancer
  23. MicroRNA-16 affects key functions of human endothelial progenitor cells.
    Authors: Goretti E, Rolland-Turner M, Leonard F, Zhang L, Wagner D, Devaux Y
    J Leukoc Biol, 2013-01-16;93(5):645-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Differential Effects of VEGFR-1 and VEGFR-2 Inhibition on Tumor Metastases Based on Host Organ Environment
    Authors: Yoon-Jin Lee, Daniel L. Karl, Ugwuji N. Maduekwe, Courtney Rothrock, Sandra Ryeom, Patricia A. D'Amore et al.
    Cancer Research
  26. Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
    Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
    Invest. Ophthalmol. Vis. Sci., 2009-10-15;51(2):1190-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Analysis of pairwise cell interactions using an integrated dielectrophoretic‐microfluidic system
    Authors: Zhizhong Yin, David Noren, C Joanne Wang, Rob Hang, Andre Levchenko
    Molecular Systems Biology
  28. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008-03-04;32(8):1244-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
    Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
    Biochem. Biophys. Res. Commun., 2008-02-07;368(3):543-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  32. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007-04-19;25(8):1862-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
    Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
    Blood, 2006-10-12;109(4):1479-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells.
    Authors: Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR
    Biochem. Biophys. Res. Commun., 2006-06-12;346(4):1200-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human VEGFR2/KDR/Flk-1 Antibody

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Human VEGFR2/KDR/Flk-1 Antibody
By Anonymous on 07/13/2024
Application: Flow Sample Tested: retinal cells Species: Human
Flow Cytometry Human VEGFR2/KDR/Flk-1 Antibody MAB3572
Human VEGF R2/KDR/Flk-1 Antibody
By Anonymous on 08/19/2016
Application: ELISA Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
ELISA Human VEGF R2/KDR/Flk-1 Antibody MAB3572