Human VEGFR1/Flt-1 Variant Flt1-14 Antibody

Name: Human VEGFR1/Flt-1 Variant Flt1-14 Antibody
Brand: R&D Systems
SKU: MAB6564
Price: 415 USD

Catalog #: MAB6564 Datasheet / COA / SDS

Discontinued Product

MAB6564 has been discontinued.

View all VEGFR1/Flt-1 products.

Detection of Human VEGFR1/Flt‑1 Variant Flt1-14 by Western Blot.

1 Image

All VEGFR1/Flt-1 Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR1/Flt-1 Variant Flt1-14 Antibody Summary

Species Reactivity

Human

Specificity

Detects synthetic peptide (aa 706-721) from human VEGFR1/Flt-1 Variant Flt1-14 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 611926

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human VEGFR1/Flt-1 Variant Flt1-14 synthetic peptide
Glu706-Leu721
Accession # ACA62948

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human VEGFR1/Flt‑1 Variant Flt1-14 by Western Blot. Western blot shows human serum (preeclampsia). PVDF Membrane was probed with 2 µg/mL of Human VEGFR1/Flt-1 Variant Flt1-14 Monoclonal Antibody (Catalog # MAB6564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VEGFR1/Flt-1 Variant Flt1-14 at approximately 90 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 10.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

Human VEGFR1/Flt-1 is a type I transmembrane receptor for the angiogenic cytokine, VEGF. Alternate splicings of human VEGFR1 mRNA encode soluble, secreted isoforms that can function as inhibitors of VEGF. One, designated sFlt1-14 or sFlt1-e15a, encodes a 733 amino acid (aa), 95-135 kDa form that is produced by non-endothelial cells, notably by degenerative syncytiotrophoblasts within syncytial knots in the placenta. This form appears to be unique to primates and is produced in excess during preeclampsia of pregnancy. The peptide used as an immunogen is not present within either the full-length or alternate secreted forms of the protein.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1