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Human VEGFR2/KDR/Flk-1 PerCP-conjugated Antibody

Catalog #: FAB357C
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB357C-100
FAB357C-025
Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R2/KDR/Flk-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 PerCP-conjugated Mono-clonal Antibody (Catalog # FAB357C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. CYP4A/20-HETE regulates ischemia-induced neovascularization via its actions on endothelial progenitor and preexisting endothelial cells
    Authors: Li Chen, Samantha Tang, Frank F. Zhang, Victor Garcia, John R. Falck, Michal L. Schwartzman et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  2. Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
    Authors: S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari
    Redox Biol, 2019-04-10;24(0):101192.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Circulating progenitor and angiogenic cell frequencies are abnormally static over pregnancy in women with preconception diabetes: A pilot study
    Authors: PD Lima, Z Chen, A Tayab, MS Murphy, J Pudwell, GN Smith, BA Croy
    PLoS ONE, 2017-03-09;12(3):e0172988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls
    Authors: B Wilde
    Arthritis Res Ther, 2016-06-23;18(0):147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Effects of age on noninvasive assessments of vascular function in nonhuman primates: implications for translational drug discovery.
    Authors: Knight D, Smith A, Schroeder R, Huang C, Beebe D, Sokolowski S, Wang M
    J Transl Med, 2013-04-22;11(0):101.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Plasma
    Applications: Flow Cytometry
  6. Klotho endows hepatoma cells with resistance to anoikis via VEGFR2/PAK1 activation in hepatocellular carcinoma.
    Authors: Chen L, Liu H, Liu J, Zhu Y, Xu L, He H, Zhang H, Wang S, Wu Q, Liu W, Liu Y, Pan D, Ren S, Xu J, Gu J
    PLoS ONE, 2013-03-13;8(3):e58413.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
    Authors: Cheung C, Bernardo AS, Trotter MW
    Nat. Biotechnol., 2012-01-15;30(2):165-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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