Human VSIG1 Antibody

Catalog #: MAB48181 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB48181-100
MAB48181-SP

Detection of Human VSIG1 by Western Blot.

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All VSIG1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VSIG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG-1 in direct ELISAs.

Source

Monoclonal Rabbit IgG Clone # 1314D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human VSIG-1
Val22-Gly234
Accession # Q86XK7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

1-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human VSIG1 by Western Blot. Western blot shows lysates of human stomach tissue and human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48181) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for VSIG1 at approximately 55-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of VSIG1 in HEK293 Human Cell Line Transfected with Human VSIG1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48181) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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VSIG1 in Human Testis. VSIG1 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48181) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG1

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.

Long Name

V-Set and Immunoglobulin Domain Containing 1

Entrez Gene IDs

340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)

Alternate Names

Cell surface A33 antigen; dJ889N15.1; Glycoprotein A34; GPA34; MGC44287,1700062D20Rik; V-set and immunoglobulin domain containing 1; V-set and immunoglobulin domain-containing protein 1; VSIG1