Human VSIG3 Antibody

Catalog #: MAB11226 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11226-100
MAB11226-SP

Detection of Human VSIG3 by Simple WesternTM.

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All VSIG3 Antibodies

Product Details

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VSIG3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG3 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2637B

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VSIG3
Gly129-Cys138
Accession # Q5DX21

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum

Simple Western

0.2 µg/mL

IMR‑32 human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Single Cell Western

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Detection of Human VSIG3 by Simple Western^TM. Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for VSIG3 at approximately 57 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot

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Detection of Human VSIG3 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG3

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Alternate Names

Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein