Human WT1 Antibody

Catalog # Availability Size / Price Qty
MAB57291-100
MAB57291-SP
Detection of WT1 in K562 Human Cell Line by Flow Cytometry.
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Human WT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human WT1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 960525
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human WT1
Val195-Thr317
Accession # P19544
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of WT1 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of WT1 in K562 Human Cell Line by Flow Cytometry. K562 Human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human WT1 Monoclonal Antibody (Catalog # MAB57291, filled histogram) or Mouse IgG1Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WT1

WT1 (Wilms' tumor protein 1; also WT33) is a 52‑54 kDa, nuclear member of the EGR C2H2‑type zinc‑finger family of proteins. Although its predicted MW is 49 kDa, it runs anomalously in SDS‑PAGE, likely due to a high proline content. It is widely expressed, being found in developing Sertoli cells, glomerular podocytes, neurons, and CD34+ stem cells. Human WT1 is 449 amino acids (aa) in length. It contains a Pro‑rich domain (aa 27‑83) and four consecutive C2H2 zinc finger regions (aa 323‑347; 353‑377; 383‑405; 414‑438). WT1 forms homodimers, and interacts with multiple molecules. Interaction with the zinc fingers generally promotes gene transcription, while N‑terminal interactions block gene transcription. There are at least two dozen splice variants. Some are combinations of deletions of aa 250‑266 and 408‑410, plus an alternate start site 68 aa upstream of the standard site, and a three aa substitution for aa 1‑147. Over aa 127‑249, human WT1 shares 98% aa identity with mouse WT1.

Long Name
Wilms Tumor 1
Entrez Gene IDs
7490 (Human); 22431 (Mouse); 24883 (Rat)
Alternate Names
AWT1; GUD; NPHS4GUD; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT33

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