Human ZAP70 Antibody

Catalog #: MAB3709 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3709
MAB3709-SP

Detection of Human ZAP70 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZAP70 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZAP70 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 521626

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ZAP70
Trp163-Cys254
Accession # P43403

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ZAP70 by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ZAP70 Monoclonal Antibody (Catalog # MAB3709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAP70

ZAP70 (zeta-chain (TCR) associated protein kinase 70 kDa), expressed primarily in T and NK cells, is a Syk family cytosolic protein tyrosine kinase that consists of two N-terminal SH2 domains and a C-terminal tyrosine kinase domain. Upon T cell receptor activation and phosphorylation of TCR ITAMs by Src family kinases, ZAP70 is recruited to phosphorylated ITAM sequences and subsequently phosphorylated on several tyrosine residues. ZAP70 has been implicated in several immune disorders. An autosomal recessive form of SCID in humans has been attributed to a homozygous mutation in the kinase domain of ZAP70. ZAP70 expression also defines an aggressive subset of chronic lymphocytic leukemia.

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Entrez Gene IDs

7535 (Human); 22637 (Mouse); 301348 (Rat)

Alternate Names

70 kDa zeta-associated protein; EC 2.7.10; EC 2.7.10.2; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP70; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD