Mouse alpha -Fetoprotein/AFP Antibody

Catalog #: MAB5369 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5369-100
MAB5369-SP

Mouse alpha ‑Fetoprotein/AFP ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse alpha -Fetoprotein/AFP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse alpha-Fetoprotein in direct ELISA.

Source

Monoclonal Rat IgG₁ Clone # 870826

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha-Fetoprotein/AFP
Lys19-Val605
Accession # P02772

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse alpha-Fetoprotein/AFP DuoSet ELISA Kit (Catalog # DY5369-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse alpha ‑Fetoprotein/AFP ELISA Standard Curve. Recombinant Mouse alpha ‑Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Alternate Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP