Mouse Cadherin-4/R-Cadherin Antibody Summary
His21-Ala731
Accession # P39038
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse Cadherin‑4/R‑Cadherin by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Cadherin-4/R-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cadherin-4/R-Cadherin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
View Larger
Cadherin‑4/R‑Cadherin in Mouse Brain. Cadherin-4/R-Cadherin was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Mouse Cadherin-4/R-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-4/R-Cadherin
Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta -cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively.
Product Datasheets
Citation for Mouse Cadherin-4/R-Cadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Patterning and folding of intestinal villi by active mesenchymal dewetting
Authors: Huycke, TR;Miyazaki, H;Häkkinen, TJ;Srivastava, V;Barruet, E;McGinnis, CS;Kalantari, A;Cornwall-Scoones, J;Vaka, D;Zhu, Q;Jo, H;DeGrado, WF;Thomson, M;Garikipati, K;Boffelli, D;Klein, OD;Gartner, ZJ;
bioRxiv : the preprint server for biology
Species: Xenograft
Sample Types: Organoids
Applications: IHC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse Cadherin-4/R-Cadherin Antibody
There are currently no reviews for this product. Be the first to review Mouse Cadherin-4/R-Cadherin Antibody and earn rewards!
Have you used Mouse Cadherin-4/R-Cadherin Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
