Home / Carbonic Anhydrase IX/CA9 / Mouse Carbonic Anhydrase IX/CA9 Antibody

Mouse Carbonic Anhydrase IX/CA9 Antibody

Catalog #: AF2344
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2344
AF2344-SP
Carbonic Anhydrase IX in Mouse Colon.
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Citations (16)
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Mouse Carbonic Anhydrase IX/CA9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than
1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9
Gln32-Asp390
Accession # Q8VHB5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IX (Catalog # 2344-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Carbonic Anhydrase IX antibody in Mouse Colon by Immunohistochemistry (IHC-Fr). View Larger

Carbonic Anhydrase IX in Mouse Colon. Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  3. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Citations for Mouse Carbonic Anhydrase IX/CA9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Ageing-related responses to antiangiogenic effects of sunitinib in atherosclerosis-prone mice
    Authors: Brian Meehan, Delphine Garnier, Alexander Dombrovsky, Karrie Lau, Esterina D’Asti, Nathalie Magnus et al.
    Mechanisms of Ageing and Development
  2. Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade
    Authors: Yi-Feng Gu, Shannon Cohn, Alana Christie, Tiffani McKenzie, Nicholas Wolff, Quyen N. Do et al.
    Cancer Discovery
  3. Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature
    Authors: John G. Facciponte, Stefano Ugel, Francesco De Sanctis, Chunsheng Li, Liping Wang, Gautham Nair et al.
    Journal of Clinical Investigation
  4. Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy
    Authors: I Uribesalgo, D Hoffmann, Y Zhang, A Kavirayani, J Lazovic, J Berta, M Novatchkov, TP Pai, RA Wimmer, V László, D Schramek, R Karim, L Tortola, S Deswal, L Haas, J Zuber, M Sz?cs, K Kuba, B Dome, Y Cao, BJ Haubner, JM Penninger
    EMBO Mol Med, 2019-06-24;0(0):e9266.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. CD44 Interacts with HIF-2? to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells
    Authors: E Johansson, ES Grassi, V Pantazopou, B Tong, D Lindgren, TJ Berg, EJ Pietras, H Axelson, A Pietras
    Cell Rep, 2017-08-15;20(7):1641-1653.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion
    Authors: M Swayampaku, PC McDonald, M Vallejo, E Coyaud, SC Chafe, A Westerback, G Venkateswa, J Shankar, G Gao, EMN Laurent, Y Lou, KL Bennewith, CT Supuran, IR Nabi, B Raught, S Dedhar
    Oncogene, 2017-07-10;36(45):6244-6261.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016-08-05;6(0):30739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice
    Authors: Gabi U Dachs
    Hypoxia (Auckl), 2016-04-08;4(0):41-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Western Blot
  9. Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
    Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A
    PLoS ONE, 2014-09-15;9(9):e106976.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
    Authors: Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A
    PLoS ONE, 2014-05-01;9(5):e96007.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2013-12-24;306(4):E443-56.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.
    Authors: Adams JR, Xu K, Liu JC, Agamez NM, Loch AJ, Wong RG, Wang W, Wright KL, Lane TF, Zacksenhaus E, Egan SE
    Cancer Res., 2011-02-15;71(7):2706-17.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. CO2 chemosensing in rat oesophagus.
    Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
    Gut, 2008-08-05;57(12):1654-64.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  15. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.
    Authors: Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG
    Nat. Cell Biol., 2008-02-24;10(3):361-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
    Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
    Gastroenterology, 2006-07-01;131(1):142-52.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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