Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Western Blot, Neutralization, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 42285
View all CCL11/Eotaxin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL11/Eotaxin
His24-Pro97
Accession # P48298

Specificity

Detects mouse CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CCL11/Eotaxin, recombinant mouse CCL7, and rhCCL21 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL11/Eotaxin Antibody

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Mouse CCL11/Eotaxin Antibody.

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Mouse CCL11/Eotaxin Antibody.

Recombinant Mouse CCL11/ Eotaxin (Catalog # 420-ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by MTT staining. Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420). The ND50 is typically 3-30 µg/mL.

CCL11/Eotaxin antibody in Rat Intestine by Immunohistochemistry (IHC-Fr).

CCL11/Eotaxin in Rat Intestine.

CCL11/Eotaxin was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts in submucosae. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse CCL11/Eotaxin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat intestine

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)

Neutralization

Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the Y3 rat myeloid cell line transfected with human CCR3. The Neutralization Dose (ND50) is typically 3-30 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse CCL11/Eotaxin.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the amino-terminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.

References

  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

Alternate Names

Eotaxin

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Gene Symbol

CCL11

UniProt

P48298

Additional CCL11/Eotaxin Products

Product Documents for Mouse CCL11/Eotaxin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL11/Eotaxin Antibody

For research use only

Citations for Mouse CCL11/Eotaxin Antibody

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Protocols

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