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Mouse CCL5/RANTES Antibody

Catalog #: MAB478
28 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB478-SP
MAB478-100
MAB478-500
CCL5/RANTES in Mouse Splenocytes.
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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL5/RANTES Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL5/RANTES in direct ELISAs. Shows 50% cross-reactivity with recombinant human (rh) CCL5, and no cross-reactivity with recombinant feline CCL5, rhCCL3, recombinant mouse (rm) CCL3, rmCCL4, rhCCL4, rhCCL14, or rhCCL18.
Source
Monoclonal Rat IgG2A Clone # 53405
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.025 µg/mL Recombinant Mouse CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CCL5/RANTES antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. View Larger

Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND50 is typically 0.1-0.5 µg/mL.

Immunohistochemistry Detection of Mouse CCL5/RANTES by Immunohistochemistry View Larger

Detection of Mouse CCL5/RANTES by Immunohistochemistry Antagonizing RANTES within CNS extends the survival of mice after TBEV infection. Mice were treated with Met-RANTES, vehicle, anti-RANTES mAb, or isotype-matched control Ab daily from day 2 to 8 after a primary TBEV infection. a Met-RANTES-treated mice exhibited a delay in mortality following lethal TBEV challenge. Mortality in each group was monitored daily for 14 days. Data were pooled from three independent experiments (total of approximately 18 mice per group). Statistical differences were evaluated using the Kaplan–Meier test for mortality. *P < 0.05, compared to vehicle-treated mice. b Anti-RANTES mAb-treated mice exhibited a delay in mortality following lethal TBEV challenge. Mortality in each group was monitored daily for 14 days. Data were pooled from three independent experiments (total of approximately 18 mice per group). *P < 0.05, compared to isotype Ab-treated mice. c, d Body weight changes of mice were monitored daily. For each time point, the measured values are the average of the surviving mice. Bars represent the means ± the standard deviations of three independent experiments (total of approximately 18 mice per group). e Infectious virus in brain tissues. No significant difference in virus titers was observed at day 2, 5, or 8 between Met-RANTES-treated mice and vehicle-injected mice or anti-RANTES mAb-treated and isotype Ab-injected mice. Bars represent the means ± the standard deviations of three independent experiments (n = 4 mice per group). NS not significant. f Met-RANTES or anti-RANTES mAb treatment reduced inflammatory cell accumulation (indicated by black arrows) in cerebral cortex sections, compared to results for vehicle- or isotype Ab-treated mice, respectively. The histopathological changes of PFA-fixed sections in cerebral cortex were examined by HE staining on day 8 p.i. The photomicrographs demonstrate representative images obtained from three independent experiments (n = 4 mice per group). Bars, 100 μm Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0665-9), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% amino acid (aa) sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References
  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Heeger, P. et al. (1992) Kidney Int. 41:220.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and R.C. Gallo (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Mouse CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils
    Authors: Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF;
    Cell reports
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  2. Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner
    Authors: Tang, T;Huang, X;Lu, M;Zhang, G;Han, X;Liang, T;
    Nature communications
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
    Authors: Zhichong Wu, Kevin Thierry, Sophie Bachy, Xinyi Zhang, Pia Gamradt, Hector Hernandez‐Vargas et al.
    EMBO reports
  4. Intramyocardial injected human umbilical cord-derived mesenchymal stem cells (HucMSCs) contribute to the recovery of cardiac function and the migration of CD4+ T cells into the infarcted heart via CCL5/CCR5 signaling
    Authors: Jing Liu, Xiaoting Liang, Mimi Li, Fang Lin, Xiaoxue Ma, Yuanfeng Xin et al.
    Stem Cell Research & Therapy
  5. Intratumoral gamma δ T‐Cell Infiltrates, Chemokine (C‐C Motif) Ligand 4/Chemokine (C‐C Motif) Ligand 5 Protein Expression and Survival in Patients With Hepatocellular Carcinoma
    Authors: Na Zhao, Hien Dang, Lichun Ma, Sean P. Martin, Marshonna Forgues, Kris Ylaya et al.
    Hepatology
  6. MYC inhibition reprograms tumor immune microenvironment by recruiting T lymphocytes and activating the CD40/CD40L system in osteosarcoma
    Authors: Kuo Jiang, Qianfeng Zhang, Yong Fan, Jia Li, Jitao Zhang, Wentao Wang et al.
    Cell Death Discovery
  7. Immune deconvolution and temporal mapping identifies stromal targets and developmental intervals for abrogating murine low-grade optic glioma formation
    Authors: Amanda de Andrade Costa, Jit Chatterjee, Olivia Cobb, Elizabeth Cordell, Astoria Chao, Suzanne Schaeffer et al.
    Neuro-Oncology Advances
  8. Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity
    Authors: W Seo, K Shimizu, S Kojo, A Okeke, T Kohwi-Shig, SI Fujii, I Taniuchi
    Nat Commun, 2020-03-26;11(1):1562.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Hepatospecific ablation of p38alpha MAPK governs liver regeneration through modulation of inflammatory response to CCl4-induced acute injury
    Authors: M Fortier, M Cadoux, N Boussetta, S Pham, R Donné, JP Couty, C Desdouets, S Celton-Mor
    Sci Rep, 2019-10-10;9(1):14614.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Nitrosporeusine analogue ameliorates Chandipura virus induced inflammatory response in CNS via NF?b inactivation in microglia
    Authors: AK Verma, TS Waghmare, GR Jachak, SC Philkhana, DS Reddy, A Basu
    PLoS Negl Trop Dis, 2018-07-12;12(7):e0006648.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  11. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. The HIV co-receptor CCR5 regulates osteoclast function
    Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
    Nat Commun, 2017-12-20;8(1):2226.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. CCL5 Promotes Resolution-Phase Macrophage Reprogramming in Concert with the Atypical Chemokine Receptor D6 and Apoptotic Polymorphonuclear Cells
    Authors: M Aswad, S Assi, S Schif-Zuck, A Ariel
    J. Immunol., 2017-07-03;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway
    J Neuroinflammation, 2016-08-30;13(1):209.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, Neutralization
  15. Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints
    Authors: M. E. Mikucki, D. T. Fisher, J. Matsuzaki, J. J. Skitzki, N. B. Gaulin, J. B. Muhitch et al.
    Nature Communications
  16. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
    Authors: Sottnik J, Dai J, Zhang H, Campbell B, Keller E
    Cancer Res, 2015-04-08;75(11):2151-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The Inflammatory Preatherosclerotic Remodeling Induced by Intermittent Hypoxia Is Attenuated by RANTES/CCL5 Inhibition
    Authors: Claire Arnaud, Pauline C. Beguin, Sylvie Lantuejoul, Jean-Louis Pepin, Christiane Guillermet, Graziano Pelli et al.
    American Journal of Respiratory and Critical Care Medicine
  18. T Cell-, Interleukin-12-, and Gamma Interferon-Driven Viral Clearance in Measles Virus-Infected Brain Tissue
    Authors: Samantha R. Stubblefield Park, Mi Widness, Alan D. Levine, Catherine E. Patterson
    Journal of Virology
  19. Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.
    Authors: Herz J, Pardo J, Kashkar H, Schramm M, Kuzmenkina E, Bos E, Wiegmann K, Wallich R, Peters PJ, Herzig S, Schmelzer E, Kronke M, Simon MM, Utermohlen O
    Nat. Immunol., 2009-06-14;10(7):761-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
    Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
    J. Immunol., 2009-03-01;182(5):3055-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008-08-11;205(9):2075-84.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Joint capsule treatment with enkephalin-encoding HSV-1 recombinant vector reduces inflammatory damage and behavioural sequelae in rat CFA monoarthritis.
    Authors: Lu Y, McNearney TA, Wilson SP, Yeomans DC, Westlund KN
    Eur. J. Neurosci., 2008-03-01;27(5):1153-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  25. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004-06-01;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  26. NKT cell-derived RANTES recruits APCs and CD8+ T cells to the spleen during the generation of regulatory T cells in tolerance.
    Authors: Faunce DE, Stein-Streilein J
    J. Immunol., 2002-07-01;169(1):31-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  27. Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice.
    Authors: Kovacic JC, Gupta R, Lee AC, Ma M, Fang F, Tolbert CN, Walts AD, Beltran LE, San H, Chen G, St Hilaire C, Boehm M
    J. Clin. Invest., 2009-12-28;120(1):303-14.
  28. A new HIF-1alpha/RANTES driven pathway to hepatocellular carcinoma mediated by germline haploinsufficiency of SART1/HAF.
    Authors: Koh MY, Gagea M, Sargis T et al.
    Hepatology.

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Reviews for Mouse CCL5/RANTES Antibody

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Mouse CCL5/RANTES Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Worked as capture IgG

ELISA Mouse CCL5/RANTES Antibody MAB478
Mouse CCL5/RANTES Antibody
By Anonymous on 08/12/2019
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse CCL5/RANTES Antibody MAB478
Mouse CCL5/RANTES Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

The antibody BAF478 was used with this antibody to create an ELISA to measure RANTES in mouse samples.

ELISA Mouse CCL5/RANTES Antibody MAB478