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Mouse CCL5/RANTES Antibody

Catalog #: AF478
41 Citations Datasheet / COA / SDS
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AF478
AF478-SP
Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody.
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Citations (41)
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Mouse CCL5/RANTES Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL5/RANTES in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL5/RANTES (Catalog # 478-MR)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.07-0.3 µg/mL in the presence of 0.025 µg/mL Recombinant Mouse CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. View Larger

Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/ RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND50 is typically 0.07-0.3 µg/mL.

Immunocytochemistry CCL5/RANTES antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References
  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Heeger, P. et al. (1992) Kidney Int. 41:220.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Mouse CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Rapid functional impairment of natural killer cells following tumor entry limits anti-tumor immunity
    Authors: Dean, I;Lee, CYC;Tuong, ZK;Li, Z;Tibbitt, CA;Willis, C;Gaspal, F;Kennedy, BC;Matei-Rascu, V;Fiancette, R;Nordenvall, C;Lindforss, U;Baker, SM;Stockmann, C;Sexl, V;Hammond, SA;Dovedi, SJ;Mjösberg, J;Hepworth, MR;Carlesso, G;Clatworthy, MR;Withers, DR;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization
    Authors: Liu H, Zhang L, Liu Z et al.
    iScience
  3. Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression
    Authors: Andre AB, Rees KP, O'Connor S et al.
    Frontiers in cell and developmental biology
  4. Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors
    Authors: A Vorontsova, TJ Cooper, J Haj-Shomal, M Benguigui, S Levin, B Manobla, R Menachem, M Timaner, Z Raviv, Y Shaked
    Oncogene, 2023-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Increased Adipose Tissue Expression of IL-23 Associates with Inflammatory Markers in People with High LDL Cholesterol
    Authors: S Kochumon, A Hasan, F Al-Rashed, S Sindhu, R Thomas, T Jacob, A Al-Sayyar, H Arefanian, A Al Madhoun, E Al-Ozairi, F Alzaid, HA Koistinen, F Al-Mulla, J Tuomilehto, R Ahmad
    Cells, 2022-09-29;11(19):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF kappa B activation
    Authors: Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae-I Moon, Eunji Lee et al.
    Cellular and Molecular Life Sciences
  7. RGMa regulates CCL5 expression via the BMP receptor in experimental autoimmune encephalomyelitis mice and endothelial cells
    Authors: S Tang, B Su, T Tao, W Yan, R Zhang, X Qin, J Feng
    Molecular Medicine Reports, 2022-01-14;25(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer
    Authors: J Wang, Y Zhang, Y Xiao, X Yuan, P Li, X Wang, Y Duan, VL Seewaldt, D Yu
    American journal of cancer research, 2021-05-15;11(5):2005-2024.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. CCR5 maintains macrophages in the bone marrow and drives hematopoietic failure in a mouse model of severe aplastic anemia
    Authors: AN Seyfried, A McCabe, JNP Smith, LM Calvi, KC MacNamara
    Leukemia, 2021-03-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  11. The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury
    Authors: K Yagura, H Ohtaki, T Tsumuraya, A Sato, K Miyamoto, N Kawada, K Suzuki, M Nakamura, K Kanzaki, K Dohi, M Izumizaki, Y Hiraizumi, K Honda
    PLoS ONE, 2020-03-10;15(3):e0230080.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T�cells
    Authors: M William, LP Leroux, V Chaparro, TE Graber, T Alain, M Jaramillo
    Eur. J. Immunol., 2019-05-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell Mediated Hepatitis via Compensatory Up-regulation of CXCR2 Related Chemokine Activity
    Authors: L Chen, J Gu, Y Qian, M Li, Y Qian, M Xu, J Li, Y Wen, L Xia, J Li, Q Xia, X Kong, H Wu
    Cell Mol Gastroenterol Hepatol, 2019-01-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy
    Authors: N Suffee, C Le Visage, H Hlawaty, R Aid-Launai, V Vanneaux, J Larghero, O Haddad, O Oudar, N Charnaux, A Sutton
    Sci Rep, 2017-10-16;7(1):13294.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Valproate hampers podocyte acquisition of immune phenotypes via intercepting the GSK3? facilitated NFkB activation
    Authors: P Wang, S Zhou, Y Ge, M Lu, Z Liu, R Gong
    Oncotarget, 2017-08-03;8(51):88332-88344.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Autophagy gene FIP200 in neural progenitors non-cell autonomously controls differentiation by regulating microglia
    Authors: C Wang, S Yeo, MA Haas, JL Guan
    J. Cell Biol., 2017-06-20;216(8):2581-2596.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  18. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017-04-20;7(0):46514.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation
    Authors: YT Chu, SW Wan, YC Chang, CK Lee, BA Wu-Hsieh, R Anderson, YS Lin
    Lab. Invest, 2017-02-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease
    J Biol Chem, 2016-05-12;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-alpha and IL-1 beta, and Activation of the NF-kappa B Signaling Pathway
    Authors: Cheng Yang, Dong-Mei Zhang, Zhen-Bo Song, Ya-Qin Hou, Yong-Li Bao, Lu-Guo Sun et al.
    PLOS ONE
  22. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells.
    Authors: Herz J, Johnson K, McGavern D
    J Exp Med, 2015-06-29;212(8):1153-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
    Authors: Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J
    Endocrinology, 2015-03-02;156(5):1782-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Astrocytic TGF-beta signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection.
    Authors: Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, Koshy A, Buckwalter M
    J Immunol, 2014-05-23;193(1):139-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. The novel chemokine receptor, G-protein-coupled receptor 75, is expressed by islets and is coupled to stimulation of insulin secretion and improved glucose homeostasis
    Authors: Bo Liu, Zoheb Hassan, Stefan Amisten, Aileen J. King, James E. Bowe, Guo Cai Huang et al.
    Diabetologia
  26. RANTES has a potential to play a neuroprotective role in an autocrine/paracrine manner after ischemic stroke
    Authors: Himiko Tokami, Tetsuro Ago, Hiroshi Sugimori, Junya Kuroda, Hideto Awano, Kazuo Suzuki et al.
    Brain Research
  27. Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis.
    Authors: Mishra, Pramod K, Teale, Judy M
    PLoS Negl Trop Dis, 2013-03-14;7(3):e2099.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Stromal Targeting of Sodium Iodide Symporter Using Mesenchymal Stem Cells Allows Enhanced Imaging and Therapy of Hepatocellular Carcinoma
    Authors: Kerstin Knoop, Nathalie Schwenk, Patrick Dolp, Michael J. Willhauck, Christoph Zischek, Christian Zach et al.
    Human Gene Therapy
  29. Peptide inhibitor of CXCL4-CCL5 heterodimer formation, MKEY, inhibits experimental aortic aneurysm initiation and progression.
    Authors: Iida, Yasunori, Xu, Baohui, Xuan, Haojun, Glover, Keith J, Tanaka, Hiroki, Hu, Xiaolei, Fujimura, Naoki, Wang, Wei, Schultz, Joshua R, Turner, Court R, Dalman, Ronald L
    Arterioscler Thromb Vasc Biol, 2013-01-03;33(4):718-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Transcriptome analysis of the ependymal barrier during murine neurocysticercosis
    Authors: Pramod Kumar Mishra, Judy M Teale
    Journal of Neuroinflammation
  31. The Inflammatory Preatherosclerotic Remodeling Induced by Intermittent Hypoxia Is Attenuated by RANTES/CCL5 Inhibition
    Authors: Claire Arnaud, Pauline C. Beguin, Sylvie Lantuejoul, Jean-Louis Pepin, Christiane Guillermet, Graziano Pelli et al.
    American Journal of Respiratory and Critical Care Medicine
  32. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes.
    Authors: Laubli H, Spanaus KS, Borsig L
    Blood, 2009-09-24;114(20):4583-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
    Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
    J. Immunol., 2008-11-01;181(9):6384-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008-03-26;28(13):3277-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  36. TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
    Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
    J. Immunol., 2007-12-01;179(11):7949-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2006-10-18;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005-11-03;107(5):2112-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation
    Authors: Rubina W. Saeed, Santosh Varma, Tina Peng-Nemeroff, Barbara Sherry, David Balakhaneh, Jared Huston et al.
    The Journal of Experimental Medicine
  40. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003-05-01;71(5):2634-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  41. Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis.
    Authors: Mouton AJ, Ma Y, Rivera Gonzalez OJ et al.
    Basic Res. Cardiol.

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