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Mouse CCL9/10/MIP-1 gamma DuoSet ELISA

Catalog #: DY463
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY463
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse CCL9 / 10 / MIP-1 gamma ELISA Standard Curve
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Product Details
Procedure
Citations (11)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse CCL9/10/MIP-1 gamma DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL9/10/MIP-1gamma. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse CCL9 / 10 / MIP-1 gamma ELISA Standard Curve View Larger

Mouse CCL9 / 10 / MIP-1 gamma ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL9/10/MIP-1 gamma

Macrophage inflammatory protein 1 gamma (MIP-1 gamma), also named CCF18, is constitutively expressed in macrophage, dendritic cell and myeloid cell lines. Among CC chemokine family members, mouse MIP-1 gamma shares approximately 45%, 24% and 20% amino acid sequence identity with mouse C10, mouse MIP-1 alpha, and mouse MIP-1 beta, respectively.

Entrez Gene IDs:
20308 (Mouse)
Alternate Names:
C-C motif chemokine 9; CCF18; CCL9/10; chemokine (C-C motif) ligand 9; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2; MIP1 gamma; MIP-1 gamma; MRP-2; SCYA10; Scya9; small inducible cytokine A10; small inducible cytokine A9

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CCL9/10/MIP-1 gamma DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis
    Authors: C Hemmers, C Schulte, J Wollenhaup, DWL Wong, E Harlacher, S Orth-Alamp, BM Klinkhamme, SH Schirmer, M Böhm, N Marx, T Speer, P Boor, J Jankowski, H Noels
    Biomedicines, 2022-02-10;10(2):.
    Species: Mouse
    Sample Types: Serum
  2. Toll-like receptor 2 activation induces C-C chemokine receptor 2-dependent natural killer cell recruitment to the peritoneum
    Authors: ID Haidl, D Meghnem, TB Issekutz, JS Marshall
    Immunol. Cell Biol., 2020-09-09;0(0):.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  3. Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines
    Authors: PLD Silva, F Lauretti-F, M Caldas de, SS Lima, AE Covarrubia, M De Franco, E Carvalho, PL Ho, RMA da Costa, EAL Martins, JB Da Silva
    BMC Microbiol., 2019-01-07;19(1):4.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Chemokine CXCL16 mediates acinar cell necrosis in cerulein induced acute pancreatitis in mice
    Authors: Y Sakuma, Y Kodama, T Eguchi, N Uza, Y Tsuji, M Shiokawa, T Maruno, K Kuriyama, Y Nishikawa, Y Yamauchi, M Tsuda, T Ueda, T Matsumori, T Morita, T Tomono, N Kakiuchi, A Mima, Y Sogabe, S Marui, T Kuwada, A Okada, T Watanabe, H Nakase, T Chiba, H Seno
    Sci Rep, 2018-06-11;8(1):8829.
    Species: Mouse
    Sample Types: Serum
  5. Immune signatures of protective spleen memory CD8 T cells
    Sci Rep, 2016-11-24;6(0):37651.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Syntaxin binding protein 1 is not required for allergic inflammation via IgE-mediated mast cell activation.
    Authors: Wu Z, MacNeil A, Berman J, Lin T
    PLoS ONE, 2013-03-06;8(3):e58560.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012-09-07;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis.
    Authors: Binder NB, Niederreiter B, Hoffmann O, Stange R, Pap T, Stulnig TM, Mack M, Erben RG, Smolen JS, Redlich K
    Nat. Med., 2009-03-29;15(4):417-24.
    Species: Mouse
    Sample Types: Serum
  9. Acute brain injury triggers MyD88-dependent, TLR2/4-independent inflammatory responses.
    Authors: Koedel U, Merbt UM, Schmidt C, Angele B, Popp B, Wagner H, Pfister HW, Kirschning CJ
    Am. J. Pathol., 2007-07-01;171(1):200-13.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Effects of dexamethazone on LPS-induced activationand migration of mouse dendritic cells revealed by a genome-wide transcriptional analysis.
    Authors: Vizzardelli C, Pavelka N, Luchini A, Zanoni I, Bendickson L, Pelizzola M, Beretta O, Foti M, Granucci F, Nilsen-Hamilton M, Ricciardi-Castagnoli P
    Eur. J. Immunol., 2006-06-01;36(6):1504-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004-08-01;173(3):2084-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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