Mouse CCR3 Antibody

Catalog #: MAB729
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB729-100
MAB729-SP

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.

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All CCR3 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR3 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 83101

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and (A) Rat Anti-Mouse CCR3 Monoclonal Antibody (Catalog # MAB729) or (B) Rat IgG2A Isotype Control Antibody (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs

1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)

Alternate Names

b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citations for Mouse CCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis
Authors: HN Um, JO Baek, S Park, EH Lee, J Jang, WJ Park, JY Roh, Y Jung
Cell Death & Disease, 2021-03-04;12(3):243.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Eosinophils support adipocyte maturation and promote glucose tolerance in obesity
Authors: EH Lee, M Itan, J Jang, HJ Gu, P Rozenberg, MK Mingler, T Wen, J Yoon, SY Park, JY Roh, CS Choi, WJ Park, A Munitz, Y Jung
Sci Rep, 2018-07-02;8(1):9894.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry