Mouse CCR9 Antibody

Name: Mouse CCR9 Antibody
Brand: R&D Systems
SKU: MAB2160
Rating: 5 (1 reviews)

Catalog #: MAB2160
9 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2160-100
MAB2160-SP

Detection of CCR9 in Mouse Thymocytes by Flow Cytometry.

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All CCR9 Antibodies

Product Details

Citations (9)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 242503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR9
Met1-Leu369
Accession # Q9WUT7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB2160, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CCR9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4(+) memory T cells
Authors: BA Szilagyi, J Triebus, C Kressler, M de Almeida, S Tierling, P Durek, M Mardahl, A Szilagyi, S Floess, J Huehn, U Syrbe, J Walter, JK Polansky, A Hamann
Mucosal Immunol, 2017-02-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
Sci Rep, 2016-11-29;6(0):37914.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells.
Authors: Bakdash G, Vogelpoel L, Van Capel T, Kapsenberg M, de Jong E
Mucosal Immunol, 2014-07-16;8(2):265-78.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice.
Authors: Heidegger S, Kirchner S, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Russmann H, Endres S, Bourquin C
J Immunol, 2013-04-15;190(10):5313-20.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
E2A proteins promote development of lymphoid-primed multipotent progenitors.
Authors: Dias S, Mansson R, Gurbuxani S, Sigvardsson M, Kee BL
Immunity, 2008-07-31;29(2):217-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
J. Immunol., 2007-09-15;179(6):3724-33.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
J. Leukoc. Biol., 2006-10-19;81(1):176-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
Blood, 2006-06-29;108(8):2531-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
A genetically-encoded crosslinker screen identifies SERBP1 as a PKCepsilon substrate influencing translation and cell division
Authors: Martini S, Davis K, Faraway R Et al.
Nature communications