Mouse CCR9 Antibody

Catalog #: MAB21601 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB21601-100
MAB21601-SP

Detection of CCR9 in Mouse Thymocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR9 in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2B Clone # 242503R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR9
Met1-Leu369
Accession # Q9WUT7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB21601, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28