Mouse CD14 Biotinylated Antibody

Catalog #: BAF982
2 Citations Datasheet / COA / SDS

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BAF982

All CD14 Antibodies

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD14 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD14 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CD14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD14
Ala18-Pro345
Accession # Q4FJP7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD14 Fc Chimera (Catalog # 982-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References

Wright, S.D. et al. (1990) Science 249:1431.
Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
Beutler, B. (2000) Current Opinion in Immunology 12:20.
Stelter, F. (2000) Chem. Immunol. 74:25.

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Alternate Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CD14 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A novel role of the lumican core protein in bacterial lipopolysaccharide-induced innate immune response.
Authors: Wu F, Vij N, Roberts L, Lopez-Briones S, Joyce S, Chakravarti S
J. Biol. Chem., 2007-07-05;282(36):26409-17.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Minimally modified LDL binds to CD14, induces macrophage spreading via TLR4/MD-2, and inhibits phagocytosis of apoptotic cells.
Authors: Miller YI, Viriyakosol S, Binder CJ, Feramisco JR, Kirkland TN, Witztum JL
J. Biol. Chem., 2002-11-06;278(3):1561-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry