Mouse CD23/Fc epsilon RII Antibody

Catalog #: MAB6900 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6900
MAB6900-SP

Detection of CD23/Fc epsilon RII in Mouse Splenocytes by Flow Cytometry.

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Mouse CD23/Fc epsilon RII Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD23/Fc epsilon RII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc epsilon RIA or rhFc epsilon RIG is observed.

Source

Monoclonal Rat IgG_2A Clone # 691632

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD23/Fc epsilon RII
Glu50-Pro331
Accession # P20693

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD23/Fc epsilon RII in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD23/Fce RII Monoclonal Antibody (Catalog # MAB6900) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD23/Fc epsilon RII

The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca²⁺-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2). Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

References

Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
Sukumar, S. et al. (2006) J. Immunol. 176:4811.
Karagiannis, S.N. et al. (2001) Immunology 103:319.
Aubry, J-P. et al. (1992) Nature 358:505.
Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
Montagnac, G. et al. (2005) Traffic 6:230.
Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
Entrez Accession # EDL21949, EDL21951.
Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.

Long Name

Fc epsilon Receptor II

Entrez Gene IDs

2208 (Human); 14128 (Mouse); 171075 (Rat)

Alternate Names

BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor