Mouse CD24 APC-conjugated Antibody

Name: Mouse CD24 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8547A

Catalog #: FAB8547A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Discontinued Product

FAB8547A has been discontinued.

View all CD24 products.

Detection of CD24 in Mouse Splenocytes by Flow Cytometry.

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All CD24 Antibodies

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Mouse CD24 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD24a in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 910019

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Peptide sequence spanning a portion of the extracellular region
Accession # P24807

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD24 APC-conjugated Monoclonal Antibody (Catalog # FAB8547A) or (B) Rat IgG_2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD24

CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Alternate Names

CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen