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Mouse CD300a/LMIR1 Antibody

Catalog #: MAB1186
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1186
MAB1186-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD300a/LMIR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, approximately 2% cross-reactivity with recombinant mouse (rm) LMIR2 is observed and no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, or rmLMIR3, 4, or 5 is observed.
Source
Monoclonal Rat IgG2A Clone # 172224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
Leu28-Arg183
Accession # BAC80268
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse T1165 plasmocytoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
  5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  6. Munitz, A. et al. (2006) Blood 107:1996.
  7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
Entrez Gene IDs
11314 (Human); 217303 (Mouse)
Alternate Names
CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4

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Citations for Mouse CD300a/LMIR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation
    Authors: L Karra, R Singh Gang, R Shamri, PG Puzzovio, S Cohen-Mor, BD Levy, F Levi-Schaf
    J. Immunol., 2018-10-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Engagement Assay, Flow Cytometry
  2. CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production
    Authors: P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz
    Sci Rep, 2017-07-19;7(1):5922.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process.
    Authors: Bachelet I, Munitz A, Mankutad D, Levi-Schaffer F
    J. Biol. Chem., 2006-07-10;281(37):27190-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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