Mouse CD300b/LMIR5 Antibody

Catalog # Availability Size / Price Qty
MAB2580
MAB2580-SP
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Mouse CD300b/LMIR5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) LMIR5 is observed and no cross-reactivity with recombinant mouse (rm) LMIR3, rmLMIR4, rhLMIR1, rhLMIR2, or rhLMIR6 is observed.
Source
Monoclonal Rat IgG2B Clone # 339003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300b/LMIR5
Ile18-Tyr157
Accession # Q3U497
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD300b/LMIR5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

The LMIR members, alternatively known as CLM (CMRF-35 like molecules) multigene family members, are closely mapped in the distal region of mouse chromosome 11 and are expressed widely in leukocytes. Their encoded proteins are type I membrane proteins that contain a single extracellular Ig-like domain. These proteins either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues.

Long Name
Leukocyte Mono Ig-like Receptor 5
Entrez Gene IDs
124599 (Human); 217304 (Mouse)
Alternate Names
CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 1.13.11.6; EC 6.3.4.3; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5

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