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Mouse CD300b/LMIR5 Antibody

Catalog #: AF2580
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2580
AF2580-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD300b/LMIR5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human LMIR5 is observed and less than 5% cross‑reactivity with recombinant human (rh) LMIR1, rhLMIR2, recombinant mouse LMIR3, and rhLMIR6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300b/LMIR5
Ile18-Tyr157
Accession # Q3U497
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD300b/LMIR5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

The LMIR members, alternatively known as CLM (CMRF-35 like molecules) multigene family members, are closely mapped in the distal region of mouse chromosome 11 and are expressed widely in leukocytes. Their encoded proteins are type I membrane proteins that contain a single extracellular Ig-like domain. These proteins either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues.

Long Name
Leukocyte Mono Ig-like Receptor 5
Entrez Gene IDs
124599 (Human); 217304 (Mouse)
Alternate Names
CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 1.13.11.6; EC 6.3.4.3; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5

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Citations for Mouse CD300b/LMIR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells
    Authors: B Das, BK Neilsen, KW Fisher, D Gehring, Y Hu, DJ Volle, HS Kim, JL McCall, DL Kelly, JB MacMillan, MA White, RE Lewis
    Sci Rep, 2018;8(1):3770.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis.
    J. Immunol., 2012-07-06;189(4):1773-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development, Immunoprecipitation

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