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Mouse CD45.1 APC‑conjugated Antibody

Catalog #: FAB8374A Datasheet / COA / SDS

Discontinued Product

FAB8374A has been discontinued.
View all CD45.1 products.
Detection of CD45.1 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD45.1 APC‑conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse cells expressing the CD45.1 allotype in flow cytometry. Clone A20 does not detect the mouse CD45.2 alloantigen.
Source
Monoclonal Mouse IgG2A Clone # A20
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD45.1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD45.1 in Mouse Splenocytes by Flow Cytometry. SJL mouse splenocytes were stained with Mouse Anti-Mouse CD45.1 APC-conjugated Monoclonal Antibody (Catalog # FAB8374A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45.1

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (RIII, SJL/J, STS/A, DA).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Long Name
Cluster of Differentiation 45.1
Entrez Gene IDs
5788 (Human); 19264 (Mouse)
Alternate Names
B220; CD45 antigen; CD45.1; CD45R; EC 3.1.3.48; L-CA; LY5; Ly-5.1; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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