Mouse CD45.2 APC-conjugated Antibody

Name: Mouse CD45.2 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8448A

Catalog #: FAB8448A Datasheet / COA / SDS

Discontinued Product

FAB8448A has been discontinued.

View all CD45.2 products.

Detection of CD45.2 in BALB/c Mouse Splenocytes by Flow Cytometry.

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All CD45.2 Antibodies

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Mouse CD45.2 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse cells expressing the CD45.2 allotype in flow cytometry. Clone 104 does not detect the mouse CD45.1 alloantigen.

Source

Monoclonal Mouse IgG_2A Clone # 104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

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Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD45.2 in BALB/c Mouse Splenocytes by Flow Cytometry. BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD45.2 APC-conjugated Monoclonal Antibody (Catalog # FAB8448A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45.2

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.2 is an alloantigen of CD45 expressed by Ly5.2+ mouse strains including: AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, and 129.

References

Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.

Long Name

Cluster of Differentiation 45.2

Entrez Gene IDs

5788 (Human); 19264 (Mouse)

Alternate Names

B220; CD 45; cd45 antigen; CD45.2; CD45R; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; LCA; Leukocyte common antigen; LY 5; Ly5; Ly5.2; Ly-5.2; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase receptor type C; PTPRC; RT7.2; SCID due to PTPRC deficiency; T200 glycoprotein; T200 leukocyte common antigen; T200