Mouse CD74 Antibody

Catalog #: MAB7478 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7478
MAB7478-SP

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry.

1 Image

All CD74 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD74 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD74 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD74 is observed.

Source

Monoclonal Rat IgG₁ Clone # 829706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
Gln56-Cys215
Accession # P04441

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 Monoclonal Antibody (Catalog # MAB7478, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternative splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

References

Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
Koch, N. et al. (1987) EMBO J. 6:1677.
Riberdy, J.M. et al. (1992) Nature 360:474.
Koch, N. et al. (1991) J. Immunol. 147:2643.
Roche, P.A. et al. (1991) Nature 354:392.
Leng, L. et al. (2003) J. Exp. Med. 197:1467.
Takahashi, K. et al. (2009) Respir. Res. 10:33.
Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. USA 104:17444.
Shi, X. et al. (2006) Immunity 25:595.
Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Alternate Names

CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33