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Mouse CD74 APC-conjugated Antibody

Catalog #: FAB7478A
4 Citations Datasheet / COA / SDS
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FAB7478A
Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Mouse CD74 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD74 in ELISAs.
Source
Monoclonal Rat IgG1 Clone # 829706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
Gln56-Cys215
Accession # P04441
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD74 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 APC-conjugated Monoclonal Antibody (Catalog # FAB7478A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternate splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells (6-11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non-immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

References
  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Koch, N. et al. (1987) EMBO J. 6:1677.
  3. Riberdy, J.M. et al. (1992) Nature 360:474.
  4. Koch, N. et al. (1991) J. Immunol. 147:2643.
  5. Roche, P.A. et al. (1991) Nature 354:392.
  6. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  7. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  8. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
  9. Shi, X. et al. (2006) Immunity 25:595.
  10. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  11. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  12. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  13. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  14. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
Entrez Gene IDs
972 (Human); 16149 (Mouse); 25599 (Rat)
Alternate Names
CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33

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Citations for Mouse CD74 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD74 is a regulator of hematopoietic stem cell maintenance
    Authors: S Becker-Her, M Rozenberg, C Hillel-Kar, N Gil-Yarom, MP Kramer, A Barak, L Sever, K David, L Radomir, H Lewinsky, M Levi, G Friedlande, R Bucala, A Peled, I Shachar
    PloS Biology, 2021-03-04;19(3):e3001121.
  2. IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes
    Authors: Qiu, Z;Khalife, J;Lin, AP;Ethiraj, P;Jaafar, C;Chiou, L;Huelgas-Morales, G;Aslam, S;Arya, S;Gupta, YK;Dahia, PLM;Aguiar, RCT;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Targeting CD74 in B-cell non-Hodgkin lymphoma with the antibody-drug conjugate STRO-001
    Authors: X Li, C Abrahams, A Yu, M Embry, R Henningsen, V DeAlmeida, S Matheny, T Kline, A Yam, R Stafford, T Hallam, M Lupher, A Molina
    Oncotarget, 2023-01-12;14(0):1-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling.
    Authors: Klasen C, Ohl K, Sternkopf M, Shachar I, Schmitz C, Heussen N, Hobeika E, Levit-Zerdoun E, Tenbrock K, Reth M, Bernhagen J, El Bounkari O
    J Immunol, 2014-04-23;192(11):5273-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization

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