Mouse CD8 alpha Antibody

CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD8 alpha in Mouse Splenocytes. CD8a was detected in mouse splenocytes using Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD8 alpha by Immunohistochemistry T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD8 alpha by Flow Cytometry Endogenous T cells accumulate in K14E7 skin with an enrichment for CCR6-expressing CD4 T cells.(A) Ear skin tissue was taken from K14E7 mice in addition to control mice expressing the SIY epitope under the K14 promoter. CD4 and CD8 expression was measured on gated CD45+CD3+ cells. Data is representative of at least 4 mice/group (B) The numbers of CD4+ and CD8+ T cells in K14E7 skin or control C57 skin were enumerated per square centimetre of ear skin using flow count beads in flow cytometry. Data represents pooled mice from at least two independent experiments. (C) Gated CD3+ CD4+ T cells from the lymph node or skin of K14E7 mice were analysed for expression of the chemokine receptors, CCR6 and CCR4. The left hand panels show representative plots of isotype and CCR6 antibody staining for CD4+ T cells while the graph summarises chemokine receptor staining representative of 6 mice/group from 3 independent experiments. (D) Both K14E7 mice and C57 mice were analysed for the proliferative marker, Ki67, using intracellular staining of lymphocytes derived from the skin and inguinal lymph nodes (iLN). Naive C57 spleen cells (negative control) or C57 spleen cells treated for 3 days with PMA/Ionomycin (positive control) were also analysed. The K14E7 and C57 data represent 7 mice/group derived from 3 independent experiments while controls are spleen cells from a single mouse in 3 independent experiments. The lower right hand panels are representative plots showing isotype and Ki67 staining in K14E7 or C57 mouse skin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23469070), licensed under a CC0-1.0 license. Not internally tested by R&D Systems.

Detection of CD8 alpha by Flow Cytometry T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.