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Mouse CD8 alpha Antibody

Catalog #: MAB116
13 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB116-SP
MAB116-500
MAB116-100
CD8 alpha in Mouse Splenocytes.
2 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD8 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects both the alpha and alpha ' chains of mouse CD8 (1).
Source
Monoclonal Rat IgG2A Clone # 53-6.7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus or spleen
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunoprecipitation
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 
Cell depletion
Hathcock, K.S. (1991) Current protocols in immunology, pp. 3.4.1. Kruisbeek, A.M. (1991) Current protocols in immunology, pp. 4.1.1.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Inhibition of T Cell Function
This antibody has been used for inhibition of T cell responses to IL-2 (Takahashi, K. et al., 1992, Proc. Natl. Acad. Sci. USA 89:5557 - 5561), MHC class I (Anel, A. et al., 1996, Eur. J. Immunol. 26:2310 - 2319) or antigens (Alexander-Miller, M.A. et al., 1996, J. Exp. Med. 184:485 - 492).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD8 alpha in Mouse Splenocytes. CD8a was detected in mouse splenocytes using Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References
  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Citations for Mouse CD8 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Non-thermal Nanoelectroablation of UV-induced Murine Melanomas Stimulates an Immune Response
    Authors: Richard Nuccitelli, Kevin Tran, Kaying Lui, Joanne Huynh, Brian Athos, Mark Kreis et al.
    Pigment Cell & Melanoma Research
  2. Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression.
    Authors: Xiang W, Shi R et al.
    Nat Commun
  3. Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cells
    Authors: Yee Mon, KJ;Kim, S;Dai, Z;West, JD;Zhu, H;Jain, R;Grimson, A;Rudd, BD;Singh, A;
    Nature nanotechnology
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Eggerthella lenta augments preclinical autoantibody production and metabolic shift mimicking senescence in arthritis
    Authors: Balakrishnan, B;Luckey, D;Wright, K;Davis, JM;Chen, J;Taneja, V;
    Science advances
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy
    Authors: YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong
    J. Clin. Invest., 2019-07-16;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. CCL5-deficiency enhances intratumoral infiltration of CD8+ T cells in colorectal cancer
    Authors: S Zhang, M Zhong, C Wang, Y Xu, WQ Gao, Y Zhang
    Cell Death Dis, 2018-07-10;9(7):766.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
    Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
    Cancer Immunol Res, 2017-11-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model
    Authors: R Tanaka, F Goshima, S Esaki, Y Sato, T Murata, Y Nishiyama, D Watanabe, H Kimura
    Am J Cancer Res, 2017-08-01;7(8):1693-1703.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  9. Deficiency of the immunostimulatory cytokine IL-21 promotes intestinal neoplasia via dysregulation of the Th1/Th17 axis
    Authors: M Shapiro, B Nandi, G Gonzalez, RH Prabhala, H Mashimo, Q Huang, NY Frank, NC Munshi, JS Gold
    Oncoimmunology, 2016-12-07;6(1):e1261776.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014-04-14;9(4):e94793.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  11. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2006-11-15;81(3):1241-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. RNU (Foxn1 RNU-Nude) Rats Demonstrate an Improved Ability to Regenerate Muscle in a Volumetric Muscle Injury Compared to Sprague Dawley Rats
    Authors: Michael J. McClure, Lucas C. Olson, David J. Cohen, Yen Chen Huang, Shirley Zhang, Tri Nguyen et al.
    Bioengineering (Basel)
  13. Alteration of Adaptive Immunity in a Colorectal Peritoneal Carcinomatosis Model
    Authors: Rongchen Shi, Wei Xiang, Xia Kang, Lili Zhang, Jinping Wang, Hongming Miao et al.
    Journal of Cancer

FAQs

  1. Is Mouse CD8 alpha Antibody, Catalog # MAB116, suitable for IHC with paraffin-embedded tissue?

    • We test this antibody agasint cells and do not have data to show its use against paraffin-embedded tissue. We have citations where customers have used the clone in paraffin-embedded tissue; please see the citation link on the webpage. We are also aware the literature suggests this clone works best with acetone-fixed frozen sections or zinc-fixed paraffin sections.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539256/

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Reviews for Mouse CD8 alpha Antibody

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Mouse CD8 alpha Antibody
By Anonymous on 06/05/2018
Application: IHC on Frozen tissue Sample Tested: Liver tissue Species: Mouse
Other Mouse CD8 alpha Antibody MAB116
Mouse CD8 alpha Antibody
By Mozhdeh Sojoodi on 08/08/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreatic cancer tissue Species: Mouse

1:100 coccentration

Immunocytochemistry/Immunofluorescence Mouse CD8 alpha Antibody MAB116