Mouse CD96 PE-conjugated Antibody

Catalog #: FAB5690P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5690P

Detection of CD96 in Mouse Splenocytes by Flow Cytometry.

1 Image

All CD96 Antibodies

Product Details

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD96 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD96 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD96v2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 630612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD96
Val22-Met536
Accession # Q3U0X8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD96 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD96 PE-conjugated Monoclonal Antibody (Catalog # FAB5690P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma δ T cells. Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355). Unlike human, there is no splice variant in the second V-type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437-602. Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile