Mouse Chitinase 3-like 1/YKL-40 Antibody

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot. Western blot shows lysates of mouse lung tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB2649) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Chitinase 3-like 1/YKL-40 by Western Blot Targeted inhibition of Chi3l1 by dNP2-siChi3l1 complex enhances effector cytokine production in Th1 & CD8 T. a Schematic diagram of the complex formation of dNP2-HA2 peptide & Chi3l1 siRNA (siChi3l1) at indicated N/P ratios. b Gel retardation assay of dNP2-siChi3l1 complex. c The size of free siRNA, free peptide, & dNP2-siRNA complexes measured by Nano particle size analyzer. d Dose dependent reduction of Chi3l1 mRNA expression by dNP2-siChi3l1 complex at 1:25 N/P ratio. e Chi3l1 protein level in culture supernatants measured by ELISA. f Chi3l1 mRNA in the lung after intranasal administration of dNP2-siEGFP or dNP2-siChi3l1 complex at 1:25 N/P ratio. Chi3l1 mRNA normalized to beta -actin, & presented as relative to expression at day 0. g Chi3l1 protein level in the lung was analyzed by WBting. Chi3l1 protein relative to expression at day 0. h Densitometric values of band intensity was calculated by normalization to the value of beta -actin, then the value was represented as relative to the value at day 0. Statistical significance of dNP2-siChi3l1 treated group was analyzed to dNP2-siEGFP treated group on each day. i FACS-sorted WT naïve CD4 T differentiated into Th1 & WT naïve CD8 T activated by plate-bound anti-CD3 & anti-CD28 antibodies with IL-2 for 5 days with indicated concentrations of dNP2-siEGFP or dNP2-siChi3l1 complexes. IFN gamma & TNF alpha expression analyzed by flow cytometry. j % of TNF alpha +IFN gamma + represented as bar graph. k Chi3l1 & IFN gamma mRNA expression in dNP2-siChi3l1 treated Th1. Each gene expression level was normalized to beta -actin, & represented as fold change to those of WT Th1 cell. Data are presented as mean ± SD of three sets of independent experiments (n = 6). n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29403003), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Chitinase 3-like 1/YKL-40 by Western Blot Chitinase and Chitinase-like protein expressions in T cells. a, b mRNA expression of chitinase (Chitotriosidase, AMCase) and chitinase-like protein (Chi3l1, Ym-1) in mouse CD4 and CD8 T cells upon anti-CD3 and anti-CD28 stimulation for 3 days. Each gene expression level was normalized to beta -actin, and represented as fold change to Non-activated (NA). c, d Chi3l1 protein level in CD4 and CD8 T cells stimulated by plate-bound anti-CD3 and anti-CD28 antibodies for indicated time point. Densitometric values of band intensity were calculated by normalization to the value of beta -actin. e Chi3l1 mRNA expression in naïve and effector T cell subsets. Chi3l1 mRNA expression level was normalized to beta -actin level. Data are mean ± SD of three sets of independent experiments (n = 6). n.d., not detected; **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29403003), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Chitinase 3-like 1/YKL-40 by Western Blot Chitinase and Chitinase-like protein expressions in T cells. a, b mRNA expression of chitinase (Chitotriosidase, AMCase) and chitinase-like protein (Chi3l1, Ym-1) in mouse CD4 and CD8 T cells upon anti-CD3 and anti-CD28 stimulation for 3 days. Each gene expression level was normalized to beta -actin, and represented as fold change to Non-activated (NA). c, d Chi3l1 protein level in CD4 and CD8 T cells stimulated by plate-bound anti-CD3 and anti-CD28 antibodies for indicated time point. Densitometric values of band intensity were calculated by normalization to the value of beta -actin. e Chi3l1 mRNA expression in naïve and effector T cell subsets. Chi3l1 mRNA expression level was normalized to beta -actin level. Data are mean ± SD of three sets of independent experiments (n = 6). n.d., not detected; **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29403003), licensed under a CC-BY license. Not internally tested by R&D Systems.