Mouse Chitinase 3-like 1/YKL-40 Biotinylated Antibody

Catalog #: BAF2649
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BAF2649

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Chitinase 3-like 1/YKL-40 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Chitinase 3-like 1/YKL-40 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human Chitinase 3‑like 1/YKL-40 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Chitinase 3-like 1/YKL-40
Tyr22-Ala381
Accession # Q61362

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Chitinase 3-like 1/YKL-40

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

References

Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
Shao, R. et al. (2009) Oncogene 28:4456.
Recklies, A.D. et al. (2002) Biochem. J. 365:119.
Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
Letuve, S. et al. (2008) J Immunol. 181:5167.
Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
Johansen, J.S. et al. (1999) Rheumatology 38:618.
Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Alternate Names

AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

Product Datasheets

Product Datasheet

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Product Specific Notices

This product or the use of this product is covered by US Patent # 7,230,086 which is owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes.

Related Research Areas

Citation for Mouse Chitinase 3-like 1/YKL-40 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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