Mouse Collectrin Antibody Summary
Glu15-Ile144
Accession # Q9ESG4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Collectrin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collectrin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Collectrin in Mouse Kidney. Collectrin was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Detection of Mouse Collectrin by Simple WesternTM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Collectrin at approximately 69 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collectrin
Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta -cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.
Product Datasheets
Citation for Mouse Collectrin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Molecular basis for the interaction of the mammalian amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin.
Authors: Fairweather S, Broer A, Subramanian N, Tumer E, Cheng Q, Schmoll D, O'Mara M, Broer S
J Biol Chem, 2015-08-03;290(40):24308-25.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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