Mouse CRACC/SLAMF7 Antibody

Catalog #: MAB4628 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4628
MAB4628-SP

Detection of Mouse CRACC/SLAMF7 by Western Blot.

1 Image

All CRACC/SLAMF7 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CRACC/SLAMF7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRACC/SLAMF7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CRACC, recombinant mouse (rm) CD229, rmCD84, or rmNTB-A is observed.

Source

Monoclonal Rat IgG_2A Clone # 520911

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CRACC/SLAMF7
Ala22-Gly224
Accession # Q8BHK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse CRACC/SLAMF7 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CRACC/SLAMF7 Monoclonal Antibody (Catalog # MAB4628) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CRACC/SLAMF7 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRACC/SLAMF7

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig‑like V-set domain and one Ig‑like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%‑35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8⁺ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4‑7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).

References

Veillette, A. (2006) Immunol. Rev. 214:22.
Tovar, V. et al. (2002) Immunogenetics 54:394.
Murphy, J.J. et al. (2002) Biochem. J. 361:431.
Bouchon, A. et al. (2001) J. Immunol. 167:5517.
Lee, J.K. et al. (2007) J. Immunol. 179:4672.
Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Entrez Gene IDs

57823 (Human); 75345 (Mouse); 102133710 (Cynomolgus Monkey)

Alternate Names

19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7