Mouse CRACC/SLAMF7 Antibody

Catalog #: MAB46281
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB46281
MAB46281-SP

Detection of CRACC/SLAMF7 in Mouse Splenoctyes by Flow Cytometry.

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All CRACC/SLAMF7 Antibodies

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Mouse CRACC/SLAMF7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CRACC is observed.

Source

Monoclonal Rat IgG_2A Clone # 520914

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CRACC/SLAMF7
Ala22-Gly224
Accession # Q8BHK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CRACC/SLAMF7 in Mouse Splenoctyes by Flow Cytometry. Mouse Splenocytes were stained with Rat Anti-Mouse CRACC/SLAMF7 Monoclonal Antibody (Catalog # MAB46281, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRACC/SLAMF7

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig‑like V-set domain and one Ig‑like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%‑35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8⁺ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4‑7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).

References

Veillette, A. (2006) Immunol. Rev. 214:22.
Tovar, V. et al. (2002) Immunogenetics 54:394.
Murphy, J.J. et al. (2002) Biochem. J. 361:431.
Bouchon, A. et al. (2001) J. Immunol. 167:5517.
Lee, J.K. et al. (2007) J. Immunol. 179:4672.
Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Entrez Gene IDs

57823 (Human); 75345 (Mouse); 102133710 (Cynomolgus Monkey)

Alternate Names

19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7

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Citation for Mouse CRACC/SLAMF7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1