Mouse Cyr61/CCN1 Antibody

Name: Mouse Cyr61/CCN1 Antibody
Brand: R&D Systems
SKU: MAB4864
Rating: 4.5 (2 reviews)

Catalog #: MAB4864
2 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4864
MAB4864-SP

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Citations (2)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cyr61/CCN1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cyr61/CCN1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cyr61 is observed.

Source

Monoclonal Rat IgG_2B Clone # 466305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Cyr61/CCN1
Asp176-Gly281
Accession # P18406

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Cyr61/CCN1 in Mouse Trigeminal Ganglia. Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyr61/CCN1

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Alternate Names

CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Cyr61/CCN1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Plasminogen regulates mesenchymal stem cell-mediated tissue repair after ischemia through Cyr61 activation
Authors: H Duan, Z He, M Lin, Y Wang, F Yang, RA Mitteer, HJ Kim, E Yeo, H Han, L Qin, Y Fan, Y Gong
JCI Insight, 2020-08-06;5(15):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Functional Assay, Neutralization, Western Blot
Plasminogen Regulates Fracture Repair by Promoting the Functions of Periosteal Mesenchymal Progenitors
Authors: Luqiang Wang, Lutian Yao, Hao Duan, Fan Yang, Maohuan Lin, Rongxin Zhang et al.
Journal of Bone and Mineral Research