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Mouse Dectin-1/CLEC7A Antibody

Catalog #: MAB17561
6 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB17561
MAB17561-SP
Detection of Dectin‑1/CLEC7A in Raw264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (6)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.
Source
Monoclonal Rat IgG2A Clone # 218820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.25 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 750 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Dectin‑1/CLEC7A in Raw264.7 Mouse Cell Line by Flow Cytometry. Mouse Raw264.7 monocyte/macrophage cell line was stained with Rat Anti-Mouse Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB17561, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

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Citations for Mouse Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing
    Authors: Zhou X, Brown BA, Siegel AP et al.
    ACS Nano
  2. Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation
    Authors: J Liu, C Wu, X Li, Q Yan, MJT Reaney, Z Jiang
    Carbohydr Polym, 2019-02-23;213(0):59-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015-03-18;10(3):e0119244.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  4. A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta -Glucans
    Authors: Bilin Dong, Dongsheng Li, Ruoyu Li, Sharon C.-A. Chen, Weihuang Liu, Wei Liu et al.
    PLoS ONE
  5. Detection of a TLR2 agonist by hematopoietic stem and progenitor cells impacts the function of the macrophages they produce.
    Authors: Yanez A, Hassanzadeh-Kiabi N, Ng M, Megias J, Subramanian A, Liu G, Underhill D, Gil M, Goodridge H
    Eur J Immunol, 2013-06-07;43(8):2114-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.
    Authors: Ozment-Skelton TR, deFluiter EA, Ha T, Li C, Graves BM, Ferguson DA, Schweitzer JB, Preizsner J, Brown GD, Gordon S, Kalbfleisch JH, Williams DL
    Crit. Care Med., 2009-03-01;37(3):1038-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse Dectin-1/CLEC7A Antibody
By Anonymous on 11/01/2021
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Mouse

Detection of Mouse Dectin-1 on mouse monocytes from PBMCs of C57BL/6 mouse strain. Mouse PBMC were gated on monocytes and Dectin-1 was detected with the Mouse Dectin-1 Antibody (catalog # MAB17561) (BLUE) or Rat IgG2A isotype control (RED), followed by a secondary Goat Anti-Rat IgG APC antibody.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Flow Cytometry Mouse Dectin-1/CLEC7A Antibody MAB17561
Mouse Dectin-1/CLEC7A Antibody
By Anonymous on 07/11/2016
Application: Func Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse

72 hours cytotoxicity assay with secondary Zap conjugate

Functional Assay Mouse Dectin-1/CLEC7A Antibody MAB17561