Mouse Dectin-1/CLEC7A Antibody Summary
Phe69-Leu244
Accession # Q6QLQ4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Dectin-1/CLEC7A by Western Blot MTX amplification and characterization of sDectin-1 cell pools.(A) Western blotting of supernatant samples from cultures adapted to different MTX concentrations using an mDectin-1 goat polyclonal antibody (1∶1000; AF1756; R&D Systems) with a HRP conjugated anti-goat antibody (1∶2000; Catalog number V8051; Promega). cHis pool and nHis pool are cell pools producing sDectin-1 with histidine tagged at the C- and N-terminals respectively. (B) Shake flask batch culture cell growth and sDectin-1 production profiles of sDectin-1 producing cell pools. Values shown represent mean values obtained from three replicate flasks. Error bars indicate the standard deviation of the experiment. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse Dectin-1/CLEC7A Antibody by Western Blot OatN beta -glucan binds to HPCA, promotes microglia up-take of oatN and inhibits dectin-1 activation, whereas OatN digalactosyldiacylglycerol (DGDG) inhibits oatN beta -glucan access to dectin-1. H) A representative western blot of p-Syk in BV2 cells treated with Eth (200 mm) or Eth plus OBG (20 mg mL−1). Results represent 1 out of 3 independent experiments. Band intensity was quantified and normalized to beta -actin and the results are presented between the panels. I) The surface plasmon resonance (SPR) assay was performed to determine OBG binding to dectin-1. (Upper panel) BG (commercial beta -glucan, 100 ng mL−1), PBLP (liposomes made by peripheral blood cell derived lipids), OatN (20 mg mL−1), and OBG (20 mg mL−1) were used as analytes. (Bottom panel) OBG-DGDG (OBG remove DGDG, 20 or 60 mg mL−1) and OBG-PC (OBG remove PC, 20 mg mL−1) were used as analytes. Results represent 1 out of 3 independent experiments. J) Dectin-1 binding to HPCA-Rab11a complex was detected by immunoprecipitation. HPCA complex in BV2 cells treated with Eth or OBG was pulled down by anti-HPCA antibody (1 mg mL−1). Dectin-1 and Rab11a were probed in a western blot. Results represent 1 out of 3 independent experiments. Band intensity was quantified and normalized by HPCA, and the results are presented between the panels. K) Confocal images of dectin-1 (green) overlay with Rab11a (red) in BV2 cells treated with Eth (200 mm) or Eth plus OBG (20 mg mL−1). Results represent 1 out of 3 independent experiments. Five random fields were photographed, and representative results are shown. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34897972), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25-34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).
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- Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
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- Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
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Product Datasheets
Citations for Mouse Dectin-1/CLEC7A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The Role of AIRE in the Immunity Against Candida Albicans in a Model of Human Macrophages
Authors: JAT de Albuque, PP Banerjee, A Castoldi, R Ma, NB Zurro, LH Ynoue, C Arslanian, MUW Barbosa-Ca, JEM Correia-De, FG Weiler, MR Dias-da-Si, M Lazaretti-, LA Pedroza, NOS Câmara, E Mace, JS Orange, A Condino-Ne
Front Immunol, 2018-03-21;9(0):567.
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The Lipid Raft-Associated Protein Stomatin Is Required for Accumulation of Dectin-1 in the Phagosomal Membrane and for Full Activity of Macrophages against Aspergillus fumigatus
Authors: Marie Goldmann, Franziska Schmidt, Zoltán Cseresnyés, Thomas Orasch, Susanne Jahreis, Susann Hartung et al.
mSphere
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Role of Dectin-1 in peripheral nerve injury
Authors: Angela Yu-Huey Hsu, Sung-Tsang Hsieh
Frontiers in Cellular Neuroscience
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Restoring Oat Nanoparticles Mediated Brain Memory Function of Mice Fed Alcohol by Sorting Inflammatory Dectin‐1 Complex Into Microglial Exosomes
Authors: Fangyi Xu, Jingyao Mu, Yun Teng, Xiangcheng Zhang, Kumaran Sundaram, Mukesh K. Sriwastva et al.
Small
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Loss of TMEM 106B potentiates lysosomal and FTLD ‐like pathology in progranulin‐deficient mice
Authors: Georg Werner, Markus Damme, Martin Schludi, Johannes Gnörich, Karin Wind, Katrin Fellerer et al.
EMBO reports
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Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
EMBO Mol Med, 2019-06-01;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The dectin 1 agonist curdlan regulates osteoclastogenesis by inhibiting nuclear factor of activated T cells cytoplasmic 1 (NFATc1) through Syk kinase.
Authors: Yamasaki T, Ariyoshi W, Okinaga T, Adachi Y, Hosokawa R, Mochizuki S, Sakurai K, Nishihara T
J Biol Chem, 2014-05-12;289(27):19191-203.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Production of Functional Soluble Dectin-1 Glycoprotein Using an IRES-Linked Destabilized-Dihydrofolate Reductase Expression Vector
Authors: Say Kong Ng, Tessa Rui Min Tan, Yang Wang, Daniel Ng, Lin-Tang Goh, Muriel Bardor et al.
PLoS ONE
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The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade.
Authors: Fuller GL, Williams JA, Tomlinson MG, Eble JA, Hanna SL, Pohlmann S, Suzuki-Inoue K, Ozaki Y, Watson SP, Pearce AC
J. Biol. Chem., 2007-03-05;282(17):12397-409.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Histoplasma capsulatum alpha-(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor.
Authors: Rappleye CA, Eissenberg LG, Goldman WE
Proc. Natl. Acad. Sci. U.S.A., 2007-01-16;104(4):1366-70.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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