Mouse Dkk-1 Antibody Summary
Ser30-His272
Accession # O54908
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Dkk‑1 in Mouse Embryo. Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk‑1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dkk-1
Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.
Product Datasheets
Citations for Mouse Dkk-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Role of Klotho and AGE/RAGE-Wnt/beta-Catenin Signalling Pathway on the Development of Cardiac and Renal Fibrosis in Diabetes
Authors: B Martín-Car, J Martín-Vír, S Fernández-, A Fernández-, M Pérez-Bast, JF Navarro-Go, J Donate-Cor, C Mora-Ferná, AS Dusso, N Carrillo-L, S Panizo, M Naves-Díaz, JL Fernández-, JB Cannata-An, C Alonso-Mon
International Journal of Molecular Sciences, 2023-03-09;24(6):.
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Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance.
Authors: Gustafson B, Smith U
J. Biol. Chem., 2010-02-23;285(18):14031-41.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Thiazolidinediones increase the wingless-type MMTV integration site family (WNT) inhibitor Dickkopf-1 in adipocytes: a link with osteogenesis.
Authors: Gustafson B, Eliasson B, Smith U
Diabetologia, 2009-11-27;53(3):536-40.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Further upregulation of beta-catenin/Tcf transcription is involved in the development of macroscopic tumors in the colon of ApcMin/+ mice.
Authors: Oyama T, Yamada Y, Hata K, Tomita H, Hirata A, Sheng H, Hara A, Aoki H, Kunisada T, Yamashita S, Mori H
Carcinogenesis, 2008-01-19;29(3):666-72.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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