Mouse E-Cadherin APC-conjugated Antibody Summary
Asp157-Val709 (predicted)
Accession # P09803
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse E-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB7481A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Cadherin
Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) 251:1451.
Product Datasheets
Citations for Mouse E-Cadherin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics
Authors: C Guo, W Kong, K Kamimoto, GC Rivera-Gon, X Yang, Y Kirita, SA Morris
Genome Biol., 2019-05-09;20(1):90.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Impact of Microenvironment on the Synovial Sarcoma Transcriptome
Authors: H Jin, JJ Barrott, MG Cable, MJ Monument, DM Lerman, K Smith-Fry, D Nollner, KB Jones
Cancer Microenviron, 2017-04-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse E-Cadherin APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse E-Cadherin APC-conjugated Antibody and earn rewards!
Have you used Mouse E-Cadherin APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
