Mouse EMMPRIN/CD147 Antibody

Catalog #: MAB7721 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB7721
MAB7721-SP

Detection of Mouse EMMPRIN/CD147 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EMMPRIN/CD147 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse EMMPRIN/CD147 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1159A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse EMMPRIN/CD147
Ala22-Pro209
Accession # P18572

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

J774A.1 mouse reticulum cell sarcoma macrophage cell line

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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EMMPRIN/CD147 in Mouse Testis. EMMPRIN/CD147 was detected in perfusion fixed frozen sections of mouse testis using Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to spermatozoa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow Cytometry

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Detection of EMMPRIN/CD147 in J774A.1 cells by Flow Cytometry J774A.1 cells were stained with Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMMPRIN/CD147

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Entrez Gene IDs

682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)

Alternate Names

5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor