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EpCAM/TROP-1 Antibody

Mouse
EpCAM/TROP-1 Antibody

Catalog #: MAB8998 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8998
MAB8998-SP
Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry.
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Mouse
EpCAM/TROP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EpCAM/TROP-1.
Source
Recombinant Monoclonal Rat IgG2A Clone # G8.8R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
TE-71 Thymic epithelial cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Lujan, E. et al. (2015) Nature 521: 352.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EpCAM/TROP-1 antibody in 4T1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry. 4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB8998, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B).

Flow Cytometry View Larger

Detection of EpCAM/TROP‑1 in mIMCD3 Mouse Cell Line by Flow Cytometry. mIMCD3 mouse kidney cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB8998, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as EGP314 (Epithelial glycoprotein 314), TACSTD1 (tumor-associated calcium signal transducer 1) and CD326 is a 292 amino acid (aa), 40 kDa transmembrane glycoprotein composed of a 243 aa extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (1). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (2). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (3).

References
  1. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  2. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  3. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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