Home / F4/80 / Mouse F4/80 PerCP-conjugated Antibody

Mouse F4/80 PerCP-conjugated Antibody

Catalog #: FAB5580C
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB5580C has been discontinued.
View all F4/80 products.
Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F4/80 PerCP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse F4/80/EMR1.
Source
Monoclonal Rat IgG2A Clone # 521204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB5580C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Entrez Gene IDs
13733 (Mouse); 316137 (Rat)
Alternate Names
ADGRE1; cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; EMR1 hormone receptor; Emr1; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7L; TM7LN3

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Citation for Mouse F4/80 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activated CD8+ T Cells Cause Long-Term Neurological Impairment after Traumatic Brain Injury in Mice
    Authors: M Daglas, DF Draxler, H Ho, F McCutcheon, A Galle, AE Au, P Larsson, J Gregory, F Alderuccio, M Sashindran, RL Medcalf
    Cell Rep, 2019-10-29;29(5):1178-1191.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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