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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

Catalog #: AF1460
21 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1460
AF1460-SP
Detection of Mouse Fc gamma RII/RIII (CD32/CD16) by Western Blot.
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Citations (21)
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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Fc gamma RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # P08101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
RAW 264.7 mouse monocyte/macrophage cell line
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse Fc gamma RII/RIII (CD32/CD16) by Western Blot. Western Blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RII/RIII (CD32/CD16) at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Fc? RII/RIII (CD32/CD16) antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Fc gamma RII/RIII (CD32/CD16) in Mouse Splenocytes. Fc gamma RII/RIII (CD32/CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name
Fc gamma Receptor II/III
Alternate Names
CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A; CD32/CD16; Fc gamma RII/RIII

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Citations for Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation
    Authors: Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al.
    Frontiers in Physiology
  2. Angiocrine Wnt signaling controls liver growth and metabolic maturation in mice
    Authors: Thomas Leibing, Cyrill Géraud, Iris Augustin, Michael Boutros, Hellmut G. Augustin, Jürgen G. Okun et al.
    Hepatology
  3. Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice
    Authors: Jessica Krzistetzko, Cyrill Géraud, Christof Dormann, Anna Riedel, Thomas Leibing
    International Journal of Molecular Sciences
  4. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
    Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
    iScience
  5. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration
    Authors: Baba T, Miyazaki D, Inata K et al.
    Elife
  6. Tanshinone IIA Protects Against Cerebral Ischemia Reperfusion Injury by Regulating Microglial Activation and Polarization via NF-kappa B Pathway
    Authors: Zhibing Song, Jingjing Feng, Qian Zhang, Shanshan Deng, Dahai Yu, Yuefan Zhang et al.
    Frontiers in Pharmacology
  7. Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis
    Authors: Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C;
    Cancers
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis
    Authors: Antwi, MB;Dumitriu, G;Simón-Santamaria, J;Romano, JS;Li, R;Smedsrød, B;Vik, A;Eskild, W;Sørensen, KK;
    PloS one
    Species: Transgenic Mouse, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Cells
    Applications: Western Blot, IHC
  9. Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern
    Authors: Dietsch, B;Weller, C;Sticht, C;de la Torre, C;Kramer, M;Goerdt, S;G�raud, C;Wohlfeil, SA;
    BMC cancer
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Motor neuron survival is associated with reduced neuroinflammation and increased autophagy after brachial plexus avulsion injury in aldose reductase-deficient mice
    Authors: K Zhong, Y Huang, PLM Zilundu, Y Wang, Y Zhou, G Yu, R Fu, SK Chung, Y Tang, X Cheng, L Zhou
    Journal of Neuroinflammation, 2022-11-09;19(1):271.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. SENP6 induces microglial polarization and neuroinflammation through de-SUMOylation of Annexin-A1 after cerebral ischaemia-reperfusion injury
    Authors: M Mao, Q Xia, GF Zhan, QJ Chu, X Li, HK Lian
    Cell & bioscience, 2022-07-22;12(1):113.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment
    Authors: SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud
    Journal of Translational Medicine, 2022-02-02;20(1):62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  13. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation
    Authors: J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch
    Nature Communications, 2021-11-29;12(1):6963.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice
    Authors: Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang
    Aging (Albany NY), 2020-10-16;12(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  15. FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages
    Authors: D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong
    J Neuroinflammation, 2020-08-31;17(1):257.
    Species: Mouse
    Sample Types: Whole Tissue, Whole Tissues
    Applications: IF, IHC
  16. Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress
    Authors: P Ren, J Chen, B Li, M Zhang, B Yang, X Guo, Z Chen, H Cheng, P Wang, S Wang, N Wang, G Zhang, X Wu, D Ma, D Guan, R Zhao
    Oxid Med Cell Longev, 2020-05-11;2020(0):3050971.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. A purified capsular polysaccharide markedly inhibits inflammatory response during endotoxic shock.
    Authors: Piccioni M, Monari C, Kenno S, Pericolini E, Gabrielli E, Pietrella D, Perito S, Bistoni F, Kozel T, Vecchiarelli A
    Infect Immun, 2012-10-22;81(1):90-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. New asthma biomarkers: lessons from murine models of acute and chronic asthma.
    Authors: Di Valentin E, Crahay C, Garbacki N, Hennuy B, Gueders M, Noel A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-21;296(2):L185-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Authors: Bruno Siqueira Mietto, Antje Kroner, Elizabeth I. Girolami, Eva Santos-Nogueira, Ji Zhang, Samuel David
    The Journal of Neuroscience
  21. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation
    Authors: Mou KJ, Shen KF, Li YL et al.
    Frontiers in aging neuroscience

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