Mouse FGF-6 Antibody

Catalog #: AF5750 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5750
AF5750-SP

All FGF-6 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGF-6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF-6 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human FGF-6 and less than 1 % cross-reactivity with recombinant mouse FGF-7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse FGF-6
Pro39-Ile208
Accession # NP_034334

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature mouse FGF-6 is a 171 aa protein that shares 94% and 99% aa sequence identity with human and rat FGF-6, respectively (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5 - 7). FGF-6 expression is restricted to skeletal muscle during development, although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References

Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
de Lapeyriere, O. et al. (1990) Oncogene 5:823.
Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
Asada, M. et al. (1999) Growth Factors 16:293.
Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
de Lapeyriere, O. et al. (1993) Development 118:601.
Li, J. et al. (1993) Cancer 72:2253.
Floss, T. et al. (1997) Genes Dev. 11:2040.
Grass, S. et al. (1996) Development 122:141.

Long Name

Fibroblast Growth Factor 6

Entrez Gene IDs

2251 (Human); 14177 (Mouse); 170700 (Rat)

Alternate Names

FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2