Home / FIZZ1/RELM alpha / Mouse FIZZ1/RELM alpha Antibody

Mouse FIZZ1/RELM alpha Antibody

Catalog #: AF1523
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1523
AF1523-SP
Product Details
Citations (7)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FIZZ1/RELM alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FIZZ1/RELM alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse Resistin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FIZZ1/RELM alpha
Asp24-Ser111
Accession # Q9EP95
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse FIZZ1/RELM alpha

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FIZZ1/RELM alpha

RELM alpha, also known as ‘found in inflammatory zone 1’ (FIZZ1) belongs to the RELM1/FIZZ family of cysteine rich secretory proteins. RELM alpha is most abundantly expressed in white adipose tissue but not in preadipocytes nor 3T3-L1 adipocytes. RELM alpha expression is also detected in heart, lung, and tongue (1).

References
  1. Steppan, C.M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:502.
Long Name
Found in Inflammatory Zone 1/Resistin-like Molecule alpha
Entrez Gene IDs
84666 (Human); 57262 (Mouse)
Alternate Names
1810019L16Rik; FIZZ1; Fizz-1; HIMF; RELM alpha; RELMa; RELMalpha; RELM-alpha; Retnla resistin like alpha; RETNLA; RETNLB; Xcp2

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Citations for Mouse FIZZ1/RELM alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Human Resistin Induces Cardiac Dysfunction in Pulmonary Hypertension
    Authors: Q Lin, S Kumar, U Kariyawasa, X Yang, W Yang, JT Skinner, WD Gao, RA Johns
    Journal of the American Heart Association, 2023-03-16;12(6):e027621.
  2. In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infection
    Authors: A Dumigan, O Cappa, B Morris, J Sá Pessoa, R Calderon-G, G Mills, R Lancaster, D Simpson, A Kissenpfen, JA Bengoechea
    Embo Molecular Medicine, 2022-11-07;0(0):e16888.
    Species: Complex Species Category
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. MEK1/2 Inhibition Promotes Macrophage Reparative Properties
    Authors: Matthew E Long
    J. Immunol, 2016-12-21;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELMalpha) in chronic hypoxia- and antigen-mediated pulmonary vascular remodeling.
    Authors: Angelini D, Su Q, Yamaji-Kegan K, Fan C, Skinner J, Poloczek A, El-Haddad H, Cheadle C, Johns R
    Respir Res, 2013-01-04;14(0):1.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  5. Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members.
    Authors: Fan C, Johns B, Su Q, Kolosova I, Johns R
    Histochem Cell Biol, 2012-10-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELMalpha) increases lung inflammation and activates pulmonary microvascular endothelial cells via an IL-4-dependent mechanism.
    Authors: Yamaji-Kegan K, Su Q, Angelini DJ, Myers AC, Cheadle C, Johns RA
    J. Immunol., 2010-10-01;185(9):5539-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELM{alpha}) induces the vascular and hemodynamic changes of pulmonary hypertension.
    Authors: Angelini DJ, Su Q, Yamaji-Kegan K, Fan C, Skinner JT, Champion HC, Crow MT, Johns RA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-01-09;296(4):L582-93.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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